Minimal chemical modification of reductive end of dextran to produce an amphiphilic polysaccharide able to incorporate onto lipid nanocapsules
Minimal chemical modification of reductive end of dextran to produce an amphiphilic polysaccharide able to incorporate onto lipid nanocapsules
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DOI：
10.1021/bc700444t
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发表时间：
2008-07-01
影响因子：
4.7
通讯作者：
Melnyk, Patricia
中科院分区：
文献类型：
--
作者：
Richard, Antoine;Barras, Alexandre;Melnyk, Patricia
为了将两亲性多糖接枝到脂质纳米胶囊上，我们提出了一种新的右旋糖酐脂化方法。脂化策略是基于在两亲性羟胺C16E20ONH2和40 kDa葡聚糖的还原端之间形成肟键。这种化学选择性反应使我们能够控制脂化位点和引入右旋糖酐分子的脂质数量。将这种新型两亲性葡聚糖包覆在脂质纳米胶囊表面。动态光散射法测定包覆效率，H-1 NMR法证实多糖的存在，电镜法观察多糖的存在。
In order to graft an amphiphilic polysaccharide to lipid nanocapsules, we present here a new method of dextran lipidation. The lipidation strategy is based on the formation of an oxime linkage between the amphiphilic hydroxylamine C16E20ONH2 and the reductive end of a 40 kDa dextran. This chemoselective reaction allows us to control the lipidation site and the number of lipid introduced on the dextran molecule. This new amphiphilic dextran was used to coat the surface of lipid nanocapsules. The coating efficiency was followed by dynamic light scattering and the presence of the polysaccharide was confirmed by H-1 NMR and observed by electronic microscopy.