The scattered distribution of actin genes in the mouse and human genomes.
The scattered distribution of actin genes in the mouse and human genomes.
复制标题
肌动蛋白基因在小鼠和人类基因组中的分散分布。
DOI：
10.1002/j.1460-2075.1982.tb01211.x
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
G. Bernardi
中科院分区：
文献类型：
--
作者：
Philippe Soriano;P. Szabo;G. Bernardi
利用仓鼠肌动蛋白基因探针，将肌动蛋白基因定位在小鼠和人类DNA的主要成分上，即构成这些基因组主体的四个片段家族上。可以检测到20多个与探针杂交的EcoRI片段；在每个成分中都发现了这些片段的不同子集。由于构成小鼠和人类DNA主要成分的片段家族来自很长的染色体片段，即等轴线，所有成分上肌动蛋白基因的存在为它们在这两个基因组中的分散提供了证据。在硫酸葡聚糖存在下，125I标记的探针与中期染色体的原位杂交证实了这种分散，表明所鉴定的29-30个肌动蛋白基因位点几乎分布在所有染色体上。此外，一些人类肌动蛋白基因可以定位在特定的染色体片段上；特别是有一个基因定位在X染色体的长臂上。最后，从重组DNA文库中分离了三个不同的小鼠肌动蛋白基因，并在这些基因附近发现了散布的重复序列。
A hamster actin cDNA probe was used to localize actin genes on the major components of mouse and human DNAs, namely on the four families of fragments forming the bulk of these genomes. Over 20 EcoRI fragments hybridizing the probe could be detected; a different subset of these fragments was found in each component. Since the fragment families forming the major components of the mouse and human DNAs derive from very long chromosomal segments, the isochores, the presence of actin genes on all components provides evidence for their dispersion in both genomes. In situ hybridization of 125I‐labeled probe to metaphase chromosomes in the presence of dextran sulfate confirmed this dispersion by showing that the 29‐30 actin gene sites so identified are distributed on almost all chromosomes. Moreover, some human actin genes could be mapped on specific chromosomal segments; in particular, one gene was localized on the long arm of the X chromosome. Finally, three different mouse actin genes were isolated from a recombinant DNA library and previously investigated interspersed repeated sequences were identified in the vicinity of these genes.