Synchronous Drosophila circadian pacemakers display nonsynchronous Ca²⁺ rhythms in vivo.
Synchronous Drosophila circadian pacemakers display nonsynchronous Ca²⁺ rhythms in vivo.
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DOI：
10.1126/science.aad3997
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发表时间：
2016-02-26
期刊：
影响因子：
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通讯作者：
Taghert PH
中科院分区：
文献类型：
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作者：
Liang X;Holy TE;Taghert PH
在果蝇中，分子钟通过约150个起搏器神经元网络控制昼夜节律行为。为了解释网络的神经元特性是如何编码时间的，我们在体内对起搏器神经元组进行了24小时的全脑钙成像。起搏器表现出细胞内Ca2+的日常节律变化，这些变化受环境线索的影响，并由分子钟计时。然而，这些节律并不同步，因为每一组都表现出自己的激活阶段。与早晨或晚上运动活动相关的起搏器组显示的Ca2+节律发生在各自行为前约4小时。神经肽PDF受体的缺失促进了Ca2+波的同步。因此，需要神经肽调节来从同步分子起搏器网络中顺序输出时间。
In Drosophila, molecular clocks control circadian rhythmic behavior through a network of ~150 pacemaker neurons. To explain how the network’s neuronal properties encode time, we performed brain-wide calcium imaging of groups of pacemaker neurons in vivo for 24 hours. Pacemakers exhibited daily rhythmic changes in intracellular Ca2+ that were entrained by environmental cues and timed by molecular clocks. However, these rhythms were not synchronous, as each group exhibited its own phase of activation. Ca2+ rhythms displayed by pacemaker groups that were associated with the morning or evening locomotor activities occurred ~4 hours before their respective behaviors. Loss of receptor for neuropeptide PDF promoted synchrony of Ca2+ waves. Thus neuropeptide modulation is required to sequentially time outputs from a network of synchronous molecular pacemakers.