Immunoglobulin molecules are present in early‐generated neuronal populations in the rat cerebral cortex and retina
Immunoglobulin molecules are present in early‐generated neuronal populations in the rat cerebral cortex and retina
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DOI：
10.1002/(sici)1096-9861(19970728)384:2
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发表时间：
1997-07
影响因子：
2.5
通讯作者：
M. Upender;J. A. Dunn;S. Wilson;J. Naegele
中科院分区：
文献类型：
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作者：
M. Upender;J. A. Dunn;S. Wilson;J. Naegele
免疫球蛋白样抗原已在仅限于发育中的哺乳动物大脑皮质深层的神经元亚群中被鉴定出来。该模式提示从血清中选择性摄取免疫球蛋白（IG）或合成IG或具有IG样基序的分子。为了区分这些替代品，进行了生化，免疫细胞化学和出生日期分析。在新生大鼠大脑皮质中，免疫球蛋白样免疫反应主要位于亚板神经元、边缘区神经元和跨越皮质板的突起中。在脑室区内也观察到孤立的染色。虽然大多数染色在皮质板出生后几天不存在，subplate神经元染色持续到第二个产后周结束。定量免疫分析表明，抗原浓度从出生时皮质胞液中的130 ng/mg下降到出生后第7天（P7）的约80 ng/mg，此后保持较低水平。通过Western印迹法鉴定了两种迁移率与血清IgG重链和轻链相似的IG免疫反应性多肽。纯化较大的条带，通过Edman降解进行部分测序，发现与大鼠IgG重链匹配。溴脱氧尿苷出生日期和抗IgG双标记研究表明，大多数含IG的细胞是早期生成的神经元。在皮质外，在视网膜和小脑的神经元中也检测到瞬时IgG染色。这些研究表明，亚板和其他早期生成的神经元选择性地从血清中摄取IG。然后，IgG可能在发育调节的细胞死亡期间从中枢神经系统（CNS）降解或丢失。J. Comp.神经元384：271-282，1997.© 1997 Wiley利斯公司
Immunoglobulin‐like antigens have been identified in neuronal subsets restricted to deeper layers of the developing mammalian cerebral cortex. The pattern is suggestive of selective uptake of immunoglobulin (Ig) from serum or synthesis of Ig or a molecule with Ig‐like motifs. To distinguish between these alternatives, biochemical, immunocytochemical, and birthdating analyses were conducted. In neonatal rat cerebral cortex, immunoglobulin‐like immunoreactivity was chiefly in subplate neurons, marginal zone neuropil, and processes spanning the cortical plate. Isolated staining was also observed within the ventricular zone. Although most staining in the cortical plate was absent several days after birth, subplate neuron staining persisted until the end of the second postnatal week. Quantitative immunoassays showed that the antigen concentration dropped from 130 ng/mg in cortical cytosol at birth to approximately 80 ng/mg by postnatal day 7 (P7) and remained low thereafter. Two Ig‐immunoreactive polypeptides with mobilities similar to heavy and light chains of serum IgG, were identified by Western blotting. The larger band was purified, partially sequenced by Edman degradation, and found to match rat IgG heavy chain. Bromodeoxyuridine birthdating and anti‐IgG double‐labeling studies showed that most of the Ig‐containing cells were early‐generated neurons. Outside of the cortex, transient IgG staining was also detected in neurons of the retina and cerebellum. These studies suggest that subplate and other early‐generated neurons selectively take up Ig from serum. The IgG may then either be degraded or lost from the central nervous system (CNS) during developmentally regulated cell death. J. Comp. Neurol. 384:271–282, 1997. © 1997 Wiley‐Liss, Inc.