Peptidergic neurohemal system of an insect: Mass spectrometric morphology
Peptidergic neurohemal system of an insect: Mass spectrometric morphology
复制标题
昆虫的肽能神经血管系统：质谱形态学
DOI：
10.1002/cne.1073
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发表时间：
2001
影响因子：
2.5
通讯作者：
R. Predel
中科院分区：
文献类型：
--
作者：
R. Predel
神经肽是昆虫中种类最多的信使分子。为了理解细胞信号传导和功能，首先必须揭示单个神经元/神经/神经血管器官的完整神经肽谱。在这项研究中，基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱法用于分析昆虫的肽能系统，重点是神经血管结构。主要的神经血管器官进行了研究，包括大脑后复合体，persymptomatic器官，和所有的神经供应这些器官的神经分泌物。此外，周围神经血管的释放网站，如触角循环器官的扩张肌和侧心神经，以及部分口胃神经系统进行了研究。分析了以下神经肽家族：激肽、allatostatin、leucomyosuppressin、corazonin、脂肪运动激素、肌抑制肽、磺胺激肽、内脏周激肽、YLSamide、VEAacid、SKNacid、proctolin、头肽和焦激肽。除了对神经血管系统中神经肽分布图的贡献外，还可以从这些实验中得出以下结论。(1)不同质谱图中几乎所有丰富的峰都代表已经鉴定的肽。(2)尽管本研究中仅使用成年雄性，但观察到肽丰度的变化可能与不同的生理/发育条件相关。(3)肽在神经血液系统中具有身体区域特异性分布。(4)可以观察到大脑后复合体的明显区室化。J. Comp.神经元436：363-375，2001.© 2001 Wiley利斯公司
Neuropeptides are by far the most diverse group of messenger molecules in insects. To understand cell signaling and function, it is essential to reveal the complete neuropeptide profile of a single neuron/nerve/neurohemal organ first. In this study, matrix‐assisted laser desorption ionization time of flight (MALDI‐TOF) mass spectrometry was used to analyze the peptidergic system of an insect, focussing on the neurohemal structures. Major neurohemal organs were investigated, including the retrocerebral complex, perisympathetic organs, and all nerves supplying these organs with neurosecretions. Additionally, peripheral neurohemal release sites such as the dilator muscle of the antennal circulatory organ and lateral heart nerves were studied, as well as parts of the stomatogastric nervous system. The following neuropeptide families were analyzed: kinins, allatostatins, leucomyosuppressin, corazonin, adipokinetic hormones, myoinhibitory peptide, sulfakinins, periviscerokinins, YLSamide, VEAacid, SKNacid, proctolin, the head peptide, and pyrokinins. Beyond a contribution to a map of the distribution of neuropeptides in a neurohemal system, the following conclusions can be drawn from these experiments. (1) Nearly all abundant peaks in the different mass spectra represent peptides that have already been identified. (2) Although only adult males were used in this study, variations in the peptide abundances were observed that are possibly correlated with different physiological/developmental conditions. (3) Peptides have a body‐region–specific distribution in the neurohemal system. (4) A clear compartmentalisation of the retrocerebral complex could be observed. J. Comp. Neurol. 436:363–375, 2001. © 2001 Wiley‐Liss, Inc.
影响因子：
7.4
作者：
Chaurand, P;Stoeckli, M;Caprioli, RM
通讯作者：
Caprioli, RM
影响因子：
7.4
作者：
Li, LJ;Garden, RW;Sweedler, JV
通讯作者：
Sweedler, JV