AN ACTIN-NUCLEATING ACTIVITY IN POLYMORPHONUCLEAR LEUKOCYTES IS MODULATED BY CHEMOTACTIC PEPTIDES
AN ACTIN-NUCLEATING ACTIVITY IN POLYMORPHONUCLEAR LEUKOCYTES IS MODULATED BY CHEMOTACTIC PEPTIDES
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DOI：
10.1083/jcb.103.6.2707
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发表时间：
1986-12-01
影响因子：
7.8
通讯作者：
ZIGMOND, SH
中科院分区：
文献类型：
--
作者：
CARSON, M;WEBER, A;ZIGMOND, SH
我们检测了在趋化多肽添加后不同时间制备的多形核白细胞裂解物中肌动蛋白的成核活性。在加入多肽后，肌动蛋白的核化在15 S内增加2~3倍，在90 S内衰减到基础水平，这在很大程度上不依赖于细胞质的钙离子流量。多肽诱导的成核位点表现为游离的带刺末端，因此可能会增加体内聚合的肌动蛋白水平。新的成核位置也可能决定肌动蛋白聚合的细胞位置。肌动蛋白聚合的这种局部化对于趋化过程中片状脂蛋白的定向延伸可能是重要的。
We examined the actin-nucleating activity in polymorphonuclear leukocyte lysates prepared at various times after chemotactic peptide addition. The actin nucleation increase two- to threefold within 15 s after peptide addition, decays to basal levels within 90 s, is largely independent of cytoplasmic calcium fluxes. The peptide-induced nucleation sites behave as free barbed ends and therefore may increase the level of polymerized actin in vivo. The new nucleation sites may also determine the cellular sites of actin polymerization. This localization of actin polymerization could be important for the directional extension of lamellipodia during chemotaxis.