Expression of a smaller lecithin:retinol acyl transferase transcript and reduced retinol esterification in MCF-7 cells
Expression of a smaller lecithin:retinol acyl transferase transcript and reduced retinol esterification in MCF-7 cells
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DOI：
10.1006/bbrc.2000.3995
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发表时间：
2000-12-29
影响因子：
3.1
通讯作者：
De Luca, LM
中科院分区：
文献类型：
--
作者：
Andreola, F;Giandomenico, V;De Luca, LM
视黄酸（RA）通过一个作用于卵磷脂：视黄醇酰基转移酶（LRAT）的自调节环来调节视黄酸酯的浓度。我检测了人乳腺癌细胞中视黄醇酯化活性是否下调以及LRAT的表达是否受rar调控。正常人乳腺上皮细胞（HMEC）表达类维甲酸上调的5 kb LRAT转录本，并从h -3-视黄醇合成视黄醇酯。人癌MCF-7细胞不能表达5kb的LRAT转录物，也不能合成视黄醇酯。相反，它们表达了一个2.7 kb的LRAT转录本。两个转录本都被RA上调。RAR α 403的稳定表达减弱了RA对LRAT mRNA的上调，表明MCF-7与正常乳腺细胞相比，视黄醇酯化反应降低；这些癌细胞表达较短的（2.7 kb） LRAT转录本，类视黄醛受体参与了RA对LRAT介导的视黄醛酯合成的调控。(C) 2000年学术出版社。
Retinyl ester concentration is regulated by retinoic acid (RA) through an autoregulatory loop, which acts on lecithin:retinol acyltransferase (LRAT). me tested whether retinol esterification activity is downregulated in human mammary carcinoma cells and whether LRAT expression is RAR-regulated. Normal human mammary epithelial (HMEC) cells expressed a retinoid-upregulated 5-kb LRAT transcript and synthesized retinyl esters from H-3-retinol. Human carcinoma MCF-7 cells failed to express the 5-kb LRAT transcript and to synthesize retinyl esters. Instead, they expressed a 2.7-kb LRAT transcript. Both transcripts were upregulated by RA. Stable expression of the dominant-negative RAR alpha 403 blunted the up-regulation of LRAT mRNA by RA, We conclude that retinol esterification is decreased in MCF-7 vs normal mammary cells; that these cancer cells express a shorter (2.7 kb) LRAT transcript, and that retinoid receptors are involved in the regulation of LRAT-mediated retinyl ester synthesis by RA. (C) 2000 Academic Press.