Investigation of TLR1-9 genes and miR-155 expression in dogs infected with canine distemper
Investigation of TLR1-9 genes and miR-155 expression in dogs infected with canine distemper
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DOI：
10.1016/j.cimid.2021.101711
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发表时间：
2021-10-01
影响因子：
2
通讯作者：
Arslan, Korhan
中科院分区：
文献类型：
--
作者：
Daldaban, Fadime;Bekdik, Ilknur Karaca;Arslan, Korhan
本研究旨在确定诊断为犬瘟热的犬中 Toll 样受体 (TLR) 1-9 基因和微小 RNA (miR) -155 表达水平与血液学参数的关系。研究中，两组均采用治疗前和治疗后。借助快速检测试剂盒和实时聚合酶链反应（RT-PCR），被感染的狗被诊断出患有犬瘟热。根据研究范围内检查的基因表达水平与血液学值之间的相关系数，发现治疗前组中TLR2基因与单核细胞（MON）值之间以及TLR4基因与血小板（PLT）值之间呈正相关。 TLR3 和 TLR9 基因与红细胞 (RBC) 和血红蛋白 (HGB) 值之间存在很强的正相关性；治疗后组中TLR5基因与RBC、HGB和血细胞比容(HCT)值之间以及TLR9基因与RBC和HGB值之间存在正相关关系，而TLR1基因与MON和中性粒细胞(GRAN)值之间存在正相关关系。 TLR3 基因与 HCT 值之间以及 TLR9 基因与 MON 和 HCT 值之间。研究结论：感染犬瘟热的犬治疗后，miR-155和TLR8基因均出现统计学显着性上调（P < 0.05），且同期miR-155上调与TLR8上调呈正相关。该结果表明治疗后miR155表达上调增加了TLR8基因表达。根据这些发现，miR-155可能有潜力在临床实践中用于感染犬瘟热的狗的治疗或预后。
This study aimed to determine the relationship of toll-like receptor (TLR) 1-9 genes and microRNA (miR) -155 expression levels with hematologic parameters in dogs diagnosed with canine distemper. In the study, two groups were used pre-treatment and post-treatment. Infected dogs were diagnosed with canine distemper with the help of a rapid test kit and Real Time-Polymerase Chain Reaction (RT-PCR). Based on the correlation coefficients between the expression levels of the genes examined within the scope of the study and hematologic values, a positive correlation was found between the TLR2 gene and the monocyte (MON) value and between the TLR4 gene and the platelet (PLT) value in the pre-treatment group. A strong positive correlation was identified between TLR3 and TLR9 genes and erythrocyte (RBC) and hemoglobin (HGB) values; between TLR5 gene and RBC, HGB and hematocrit (HCT) values and between TLR9 gene and RBC and HGB values in the post-treatment group, on the other hand, a positive correlation was found between TLR1 gene and MON and neutrophil (GRAN) values; between TLR3 gene and HCT value and between TLR9 gene and MON and HCT values. The study concluded that miR-155 and TLR8 gene were upregulated at a statistically significant level (P < 0.05) Posttreatment in dogs infected with canine distemper and there was a positive correlation between the upregulation of miR-155 and the upregulation of TLR8 in the same period. This result suggests that the upregulated miR155 expression post-treatment increased TLR8 gene expression. In the light of these findings, it miR-155 may have the potential to be used in clinical practice in the treatment or prognosis of dogs infected with canine distemper.