Positive regulatory gene for temperature-controlled proteins in Escherichia coli.
Positive regulatory gene for temperature-controlled proteins in Escherichia coli.
复制标题
大肠杆菌中温度控制蛋白的正调控基因。
DOI：
10.1016/s0006-291x(81)80257-4
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发表时间：
1981
影响因子：
3.1
通讯作者：
VanBogelen,RA
中科院分区：
文献类型：
--
作者：
Neidhardt,FC;VanBogelen,RA
Escherichia coliK12的一组九种蛋白质随着生长温度的变化在稳定的水平上变化，并且在40°C以上特别丰富。大多数这些HTP(高温产生)蛋白质的身份尚不清楚；它们主要是通过它们在温度升高后10分钟内非常高的合成速率在二维聚丙烯酰胺凝胶上被识别出来的。这种刺激对某些HTP蛋白的刺激高达20倍，但在75分钟时，染色体基因的条件致命性无义突变就会取消这种刺激。有证据表明，这个调控基因htpR制造了一种激活蛋白，这是热诱导HTP蛋白所必需的。
A group of nine proteins ofEscherichia coliK12 vary in steady state level with growth temperature, and are particularly abundant above 40°C. The identities of most of these HTP (high temperature production) proteins are unknown; they are primarily recognizable on two-dimensional polyacrylamide gels by their very high rates of synthesis during the ten-minute period following a shift-up in temperature. This stimulation, as much as 20-fold for some HTP proteins, is abolished by a conditionally lethal nonsense mutation in a chromosomal gene located at 75 minutes. Evidence suggests that this regulatory gene,htpR, makes an activator protein that is required for heat induction of HTP proteins.