Activity and distribution of paxillin, focal adhesion kinase, and cadherin indicate cooperative roles during zebrafish morphogenesis
Activity and distribution of paxillin, focal adhesion kinase, and cadherin indicate cooperative roles during zebrafish morphogenesis
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DOI：
10.1091/mbc.e02-08-0537
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发表时间：
2003-08-01
影响因子：
3.3
通讯作者：
Hille, MB
中科院分区：
文献类型：
--
作者：
Crawford, BD;Henry, CA;Hille, MB
我们研究了粘着斑蛋白桩蛋白和Fak，以及细胞-细胞粘附蛋白钙粘蛋白在发育斑马鱼（Danio rerio）胚胎。钙粘蛋白在它们描绘细胞的前体中胚层中表达。体节形成的起始与Fak磷酸化的增加以及Fak、磷酸化Fak、桩蛋白和纤连蛋白在新生体节边界的积累相一致。在脊索中，钙粘蛋白在嵌入期间在细胞上表达，并且磷酸化的Fak在脊索细胞接触脊索鞘中层粘连蛋白的周向环中积累。随后，鞘中胶原纤维取向的变化表明Fak介导的粘附允许脊索纵向扩张，但不允许横向扩张，导致脊索伸长。新的观察结果表明，粘着斑激酶和桩蛋白集中在上皮包膜层的细胞-细胞粘附位点，并可能与肌动蛋白细胞骨架在上皮连接处含有钙粘蛋白。Fak在这些上皮连接处被磷酸化，但在Tyr上不被磷酸化（397），这暗示了非经典的调节机制。这些数据表明，Fak和桩蛋白可能在整合钙粘蛋白为基础的细胞粘附和整合素为基础的早期斑马鱼胚胎的形态发生过程中发挥作用。
We investigated the focal adhesion proteins paxillin and Fak, and the cell-cell adhesion protein cadherin in developing zebrafish (Danio rerio) embryos. Cadherins are expressed in presomitic mesoderm where they delineate cells. The initiation of somite formation coincides with an increase in the phosphorylation of Fak, and the accumulation of Fak, phosphorylated Fak, paxillin, and fibronectin at nascent somite boundaries. In the notochord, cadherins are expressed on cells during intercalation, and phosphorylated Fak accumulates in circumferential rings where the notochord cells contact laminin in the perichordal sheath. Subsequently, changes in the orientations of collagen fibers in the sheath suggest that Fak-mediated adhesion allows longitudinal expansion of the notochord, but not lateral expansion, resulting in notochord elongation. Novel observations showed that focal adhesion kinase and paxillin concentrate at sites of cell-cell adhesion in the epithelial enveloping layer and may associate with actin cytoskeleton at epithelial junctions containing cadherins. Fak is phosphorylated at these epithelial junctions but is not phosphorylated on Tyr(397), implicating a noncanonical mechanism of regulation. These data suggest that Fak and paxillin may function in the integration of cadherin-based and integrin-based cell adhesion during the morphogenesis of the early zebrafish embryo.