Effects of low-temperature stress on secondary metabolism in mosses exposed to simulated N deposition
Effects of low-temperature stress on secondary metabolism in mosses exposed to simulated N deposition
复制标题
低温胁迫对模拟氮沉降苔藓次生代谢的影响
DOI：
10.1080/17550874.2015.1010187
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发表时间：
2015-05-04
影响因子：
1.5
通讯作者：
Liu, Weiqiu
中科院分区：
文献类型：
--
作者：
Liu, Binyang;Lei, Chunyi;Liu, Weiqiu
背景资料：氮沉降和低温胁迫（LTS）均影响植物的次生代谢，但有关低温胁迫和氮素供应共同作用下植物次生代谢的响应，特别是苔藓植物的响应还不清楚。目的：研究低温胁迫对两种藓类植物卷叶金发藓次生代谢的影响。fuscatum和雨状灰藓（Hypnum plumaeforme）在不同硝态氮或铵态氮供应水平下的生长。研究方法：在两种藓类植物，受到不同的N的应用和LTS处理的氧化胁迫和次生代谢的指标进行了测定之前和之后的一段时间的恢复10天。结果：氮的联合应用加剧了LTS诱导的氧化应激。LTS抑制P. cirratum subsp. fuscatum，但在H.对羽状藻SOD活性不敏感。施氮诱导增加L-苯丙氨酸解氨酶活性在这两种藓类植物，但随之而来的变化在藓类植物中的苯丙烷类配置文件不同。LTS仅改变苯丙素类化合物的特征。三萜类化合物的水平也受到氮供应和LTS的影响。此外，从LTS恢复10天后，一些次级代谢产物的水平仍相对较高。结论：氮供应改变了次生代谢对LTS的反应。一些次生代谢产物可能是与苔藓低温胁迫相关的胁迫记忆物质，但存在种间差异。
Background: Both nitrogen deposition and low-temperature stress (LTS) affect the secondary metabolism of plants, but few were known about the responses of secondary metabolism to combined LTS and nitrogen supply, especially for mosses. Aims: To investigate the effects of LTS on secondary metabolism in two mosses, Pogonatum cirratum subsp. fuscatum and Hypnum plumaeforme, under varied nitrate or ammonium supply rates. Methods: Indices of oxidative stress and secondary metabolism in two mosses that were subjected to different N application and LTS treatment were measured both before and after a period of 10-day recovery. Results: LTS-induced oxidative stress was exacerbated by combined N application. LTS inhibited superoxide dismutase (SOD) activity in P. cirratum subsp. fuscatum, but in H. plumaeforme SOD activity it was insensitive. N application induced increases in L-phenylalanine ammonia-lyase activity in both mosses, but the consequent changes in phenylpropanoid profiles in the mosses differed. LTS only changed the phenylpropanoid profiles. Levels of triterpenes were also affected by both N supply and LTS. In addition, levels of some secondary metabolites remained relatively high after 10-day recovery from LTS. Conclusions: N supply altered the responses of secondary metabolism to LTS. Some secondary metabolites may be stress memory substances associated with LTS of the mosses, with between-species differences.