Mechanical Response of Mouse Cervices Lacking Decorin and Biglycan During Pregnancy.
Mechanical Response of Mouse Cervices Lacking Decorin and Biglycan During Pregnancy.
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怀孕期间缺乏核心蛋白聚糖和双糖链蛋白聚糖的小鼠宫颈的机械反应。
DOI：
10.1115/1.4054199
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Myers,Kristin
中科院分区：
文献类型：
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作者：
Lee,Nicole;Shi,Lei;ColonCaraballo,Mariano;Nallasamy,Shanmugasundaram;Mahendroo,Mala;Iozzo,RenatoV;Myers,Kristin
宫颈重塑对健康妊娠至关重要。对细胞外基质(ECM)周转的适当调节导致整个妊娠期间的重塑，将组织从僵硬的材料转变为顺应性、可伸展的粘弹性组织，为分娩做好准备。富含亮氨酸的小蛋白多糖(SLRPs)调节宫颈ECM中的结构纤维组装和整体组织材料特性。为了量化SLRPs在宫颈中的力学作用，对未怀孕和妊娠晚期的SLRPs基因敲除小鼠的整个宫颈标本进行了循环加载-卸载、坡道保持和加载到失效的力学测试。在此基础上，建立了考虑胶原纤维束波度的纤维复合材料模型，用于描述宫颈三维大变形平衡行为。在非妊娠组织中，SLRP基因敲除的宫颈与野生型组织具有相同的平衡材料特性。相比之下，载荷-破坏和坡道保持试验揭示了SLRPS的冲击断裂和随时间变化的松弛行为。在非妊娠(NP)宫颈中，核心蛋白聚糖的丢失会导致较差的破裂特性。在广泛的重塑后，所有基因的颈椎强度相似，但SLRP缺乏的组织在坡道保持期间消散应力的能力减弱。在同时失去核心蛋白和双聚糖的小鼠中，怀孕的宫颈失去了延展性、顺应性和粘弹性。这些结果表明，核心蛋白聚糖和双聚糖对于健康、重塑的宫颈的重要延伸性和粘弹性材料特性是必要的。
Cervical remodeling is critical for a healthy pregnancy. The proper regulation of extracellular matrix (ECM) turnover leads to remodeling throughout gestation, transforming the tissue from a stiff material to a compliant, extensible, viscoelastic tissue prepared for delivery. Small leucine-rich proteoglycans (SLRPs) regulate structural fiber assembly in the cervical ECM and overall tissue material properties. To quantify the SLRPs' mechanical role in the cervix, whole cervix specimens from nonpregnant and late pregnant knockout mice of SLRPs, decorin and biglycan, were subjected to cyclic load-unload, ramp-hold, and load-to-failure mechanical tests. Further, a fiber composite material model, accounting for collagen fiber bundle waviness, was developed to describe the cervix's three-dimensional large deformation equilibrium behavior. In nonpregnant tissue, SLRP knockout cervices have the same equilibrium material properties as wild-type tissue. In contrast, the load-to-failure and ramp-hold tests reveal SLRPs impact rupture and time-dependent relaxation behavior. Loss of decorin in nonpregnant (NP) cervices results in inferior rupture properties. After extensive remodeling, cervical strength is similar between all genotypes, but the SLRP-deficient tissue has a diminished ability to dissipate stress during a ramp-hold. In mice with a combined loss of decorin and biglycan, the pregnant cervix loses its extensibility, compliance, and viscoelasticity. These results suggest that decorin and biglycan are necessary for crucial extensibility and viscoelastic material properties of a healthy, remodeled pregnant cervix.