SEQUENCE-SPECIFIC SCISSION OF DNA BY RNAS LINKED TO A CHEMICAL NUCLEASE
SEQUENCE-SPECIFIC SCISSION OF DNA BY RNAS LINKED TO A CHEMICAL NUCLEASE
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DOI：
10.1038/363474a0
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发表时间：
1993-06-03
期刊：
影响因子：
64.8
通讯作者：
GORIN, MB
中科院分区：
文献类型：
--
作者：
SIGMAN, DS;CHEN, CB;GORIN, MB
RNA连接到化学核酸酶1，10-菲咯啉-铜切割双链DNA的互补序列。该切割反应适用于所有序列，并可用于测量碱基对中标记基因之间的距离，绘制转录单位的大小并确定染色体断裂点的位置。
RNAs linked to the chemical nuclease 1, 10-phenanthroline-copper cut double-stranded DNA of complementary sequence. This cleavage reaction is applicable to all sequences and can be used to measure the distance between marker genes in base pairs, map the size of a transcription unit and define positions of chromosomal breakpoints.