hpttg, a human homologue of rat pttg, is overexpressed in hematopoietic neoplasms.: Evidence for a transcriptional activation function of hPTTG
hpttg, a human homologue of rat pttg, is overexpressed in hematopoietic neoplasms.: Evidence for a transcriptional activation function of hPTTG
复制标题
DOI：
10.1038/sj.onc.1202140
复制
发表时间：
1998-10-29
期刊：
影响因子：
8
通讯作者：
Pintor-Toro, JA
中科院分区：
文献类型：
--
作者：
Domínguez, A;Ramos-Morales, F;Pintor-Toro, JA
我们已经分离出一个人的cDNA克隆编码一种新的蛋白质的22 kDa，这是一个人的大鼠癌蛋白PTTG对应。我们发现，相应的基因（hpttg）在Jurkat细胞（一种人类T淋巴瘤细胞系）和不同类型的造血系统恶性肿瘤患者的样本中过表达。序列分析表明，hPTTG具有一个氨基端碱性结构域和一个羧基端碱性结构域。亚细胞分级分离研究表明，虽然hPTTG主要是一种胞浆蛋白，但它部分位于细胞核中。此外，我们表明，酸性羧基末端区域的hPTTG作为一个反式激活域融合时，异源DNA结合域，在酵母和哺乳动物细胞。
We have isolated a human cDNA clone encoding a novel protein of 22 kDa that is a human counterpart of the rat oncoprotein PTTG. We show that the corresponding gene (hpttg) is overexpressed in Jurkat cells (a human T lymphoma cell line) and in samples from patients with different kinds of hematopoietic malignancies. Analysis of the sequence showed that hPTTG has an aminoterminal basic domain and a carboxyl-terminal! acidic domain, and that it is a proline-rich protein with several putative SH3-binding sites, Subcellular fractionation studies show that, although hPTTG is mainly a cytosolic protein, it is partially localized in the nucleus. In addition we demonstrate that the acidic carboxyl-terminal region of hPTTG acts as a transactivation domain when fused to a heterologous DNA binding domain, both in yeast and in mammalian cells.