Characterization of apatite formed on alkaline-heat-treated Ti
Characterization of apatite formed on alkaline-heat-treated Ti
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DOI：
10.1177/154405910408300606
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发表时间：
2004-06-01
影响因子：
7.6
通讯作者：
Araki, Y
中科院分区：
文献类型：
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作者：
Chosa, N;Taira, M;Araki, Y
碱性热处理钛在体内自形成磷灰石表面层。本研究的目的是材料学特性的碱性热处理钛浸泡在模拟体液（AHS-TI）的表面，并检查AHS-TI上的成骨细胞的分化行为。SEM、薄膜XRD、FTIR和XPS分析表明，AHS-TI含有1.0 μ m厚、低结晶度和[ 002]方向取向的碳酸盐磷灰石表面。将人成骨细胞样SaOS-2细胞在聚苯乙烯、钛和AHS-TI上培养，并对成骨分化相关mRNA进行RTPCR分析。AHS-TI组骨涎蛋白mRNA的表达较聚苯乙烯组和钛组明显上调（p < 0.05）。AHS-TI组骨桥蛋白和骨钙素mRNA的表达较聚苯乙烯组明显上调（p< 0.05）。结果表明，磷灰石是骨样的，并加速SaOS-2的成骨分化，表明碱热处理可能有助于更好地钛种植体与骨结合。
Alkaline-heat-treated titanium self-forms an apatite surface layer in vivo. The aim of the present study was to materialistically characterize the surface of alkaline-heat-treated titanium immersed in simulated body fluid ( AHS-TI) and to examine the differentiation behavior of osteoblasts on AHS-TI. SEM, thin-film XRD, FTIR, and XPS analyses revealed that AHS-TI contained a 1.0-mum-thick, low-crystalline, and [ 002] direction-oriented carbonate apatite surface. Human osteoblast-like SaOS-2 cells were cultured on polystyrene, titanium, and AHS-TI, and RTPCR analyses of osteogenic differentiation-related mRNAs were conducted. On AHS-TI, the expression of bone sialoprotein mRNA was upregulated as compared with that on polystyrene and titanium ( p < 0.05). On AHS-TI, the expression of osteopontin and osteocalcin mRNAs was up-regulated as compared with that on polystyrene ( p< 0.05). The results indicate that the apatite was bone-like and accelerated the osteogenic differentiation of SaOS-2, suggesting that alkaline-heat treatment might facilitate better integration of titanium implants with bone.