Quorum sensing in the dimorphic fungus Candida albicans is mediated by farnesol
Quorum sensing in the dimorphic fungus Candida albicans is mediated by farnesol
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DOI：
10.1128/aem.67.7.2982-2992.2001
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发表时间：
2001-07-01
影响因子：
4.4
通讯作者：
Nickerson, KW
中科院分区：
文献类型：
--
作者：
Hornby, JM;Jensen, EC;Nickerson, KW
二态真菌白色念珠菌的接种量效应是由细胞外群体感应分子（QSM）的产生引起的。该分子在生长形态学实验和分化实验中都可以阻止菌丝的发育，使用三种化学上不同的胚管形成触发器（GTF）： l -脯氨酸，n -乙酰氨基葡萄糖和血清（猪或胎牛）。在所有情况下，QSM的存在阻止了酵母向菌丝的转化，导致酵母积极出芽，而不影响细胞生长速度。QSM在C，白色念珠菌中表现出普遍的交叉反应性，因为菌株A72的上清液对其他5株C，白色念珠菌都有活性，反之亦然。分子量,222年,37),QSM细胞外,和生产不断增长和在一个温度范围从23到43 degreesC,大概数量成正比的CFU /毫升,生产不依赖于类型的碳源和氮源或生长介质的化学性质,和商业混合异构体(E, E)法尼醇表现出QSM活动(防止GTF的能力水平足以占所有在场的QSM活动在C语言中,白色念珠菌上清液，即50% GTF，约30至35 muM。橙花醇的活性比金合耳醇低约2倍，香叶醇（C-10）、香叶基香叶醇（C-20）和金合耳基焦磷酸都没有任何QSM活性。
The inoculum size effect in the dimorphic fungus Candida albicans results from production of an extracellular quorum-sensing molecule (QSM). This molecule prevents mycelial development in both a growth morphology assay and a differentiation assay using three chemically distinct triggers for germ tube formation (GTF): L-proline, N-acetylglucosamine, and serum (either pig or fetal bovine). In all cases, the presence of QSM prevents the yeast-to-mycelium conversion, resulting in actively budding yeasts without influencing cellular growth rates. QSM exhibits general cross-reactivity within C, albicans in that supernatants from strain A72 are active on five other strains of C, albicans and vice versa, The QSM excreted by C, albicans is farnesol (C15H26O; molecular weight, 222,37), QSM is extracellular, and is produced continuously during growth and over a temperature range from 23 to 43 degreesC, in amounts roughly proportional to the CFU/milliliter, Production is not dependent on the type of carbon source nor nitrogen source or on the chemical nature of the growth medium, Both commercial mixed isomer and (E,E)-farnesol exhibited QSM activity (the ability to prevent GTF at a level sufficient to account for all the QSM activity present in C, albicans supernatants, i.e., 50% GTF at ca, 30 to 35 muM. Nerolidol was ca, two times less active than farnesol, Neither geraniol (C-10), geranylgeraniol (C-20), nor farnesyl pyrophosphate had any QSM activity.