Naltrexone; as an efficient adjuvant in induction of Th1 immunity and protection against Fasciola hepatica infection
Naltrexone; as an efficient adjuvant in induction of Th1 immunity and protection against Fasciola hepatica infection
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DOI：
10.1016/j.exppara.2018.04.015
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发表时间：
2018-06-01
影响因子：
2.1
通讯作者：
Yaghoobi, Hajar
中科院分区：
文献类型：
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作者：
Azizi, Hakim;Mirzaeei, Hadi;Yaghoobi, Hajar
现有疗法的毒性作用是寄生虫感染传统管理方法的主要缺点。疫苗为避免这种不必要的并发症提供了一个有希望的机会。在本研究中，我们检查了一种新兴佐剂纳曲酮对抗 BALB/c 小鼠肝片形吸虫感染的免疫增强能力。将 70 只 BALB/c 小鼠分为 5 个实验组（每组 14 只），包括 1-对照（接受 PBS）、2-疫苗（用肝曲霉 E/S 抗原免疫）、3-Alum-疫苗（用 Alum 佐剂和 E/S 抗原免疫）、4-NLT-疫苗（用 NLT 佐剂和 E/S 抗原免疫）和5-Alum-NLT-疫苗（用混合 Alum-NLT 佐剂和 E/S 抗原进行免疫）。采用MTT法评估淋巴细胞刺激指数。通过ELISA方法评估IFN-γ、IL-4、IgG2a和IgG1的产生。结果表明，NLT无论是单独使用还是与明矾联合使用，都可以诱导免疫反应，产生代表Th1免疫反应的IFN-γ和IgG2a。此外，在免疫实验中使用该佐剂与脾细胞/淋巴细胞显着高的增殖反应相关。使用混合明矾-NLT佐剂在感染肝镰刀菌的小鼠攻击试验中显示出最高的保护率(73.8%)。这些发现表明 NLT 作为有效佐剂在诱导针对片形吸虫病的保护性细胞和 Th1 免疫反应方面具有潜在作用。 (C) 2018 Elsevier Inc. 保留所有权利。
Toxic effects of available therapeutics are major drawbacks for conventional management approaches in parasitic infections. Vaccines have provided a promising opportunity to obviate such unwanted complications. In present study, we examined immune augmenting capacities of an emerging adjuvant, Naltrexone, against Fasciola hepatica infection in BALB/c mice. Seventy BALB/c mice were divided into five experimental groups (14 mice per group) including 1-control (received PBS), 2-vaccine (immunized with F. hepatica E/S antigens), 3-Alum-vaccine (immunized with Alum adjuvant and E/S antigens), 4-NLT-vaccine (immunized with NLT adjuvant and E/S antigens), and 5-Alum-NLT-vaccine (immunized with mixed Alum-NLT adjuvant and E/S antigens). Lymphocyte stimulation index was assessed by MTT assay. Production of IFN-gamma, IL-4, IgG2a and IgG1 was assessed by ELISA method. Results showed that NLT, either alone or in combination with alum, can induce immune response toward production of IFN-gamma and IgG2a as representatives of Th1 immune response. Also, using this adjuvant in immunization experiment was associated with significantly high proliferative response of splenocytes/lymphocytes. Utilization of mixed Alum-NLT adjuvant revealed the highest protection rate (73.8%) in challenge test of mice infected with F. hepatica. These findings suggest the potential role of NLT as an effective adjuvant in induction of protective cellular and Th1 immune responses against fasciolosis. (C) 2018 Elsevier Inc. All rights reserved.