Cytochrome c3 from Desulfovibrio gigas: Crystal structure at 1.8 Å resolution and evidence for a specific calcium‐binding site
Cytochrome c3 from Desulfovibrio gigas: Crystal structure at 1.8 Å resolution and evidence for a specific calcium‐binding site
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来自 Desulfovibrio gigas 的细胞色素 c3：1.8 Å 分辨率的晶体结构和特定钙结合位点的证据
DOI：
10.1002/pro.5560050713
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发表时间：
1996
期刊：
影响因子：
8
通讯作者：
L. Sieker
中科院分区：
文献类型：
--
作者：
P. Matias;J. Morais;R. Coelho;M. A. Carrondo;K. Wilson;Z. Dauter;L. Sieker
从硫酸盐还原菌--长硫弧菌(Desulfovibrio gias，DG)中分离得到四氢血红素细胞色素C3(Mw 13 kDa，111个残基，4个血红素基团)的晶体，并采集了分辨率为1.8？的X射线衍射数据。用分子置换的方法对结构进行了求解，得到的模型修正为传统的R因子为14.9%。该三维结构与其他已知的四铁血红素c3细胞色素的晶体结构有许多相似之处，但也显示出一些显著的差异。特别是，在血红素I、III和IV的情况下，可能在分子的电子转移过程中发挥基本作用的血红素基团周围的芳香族残基的位置是非常保守的。然而，血红素II的芳香环境与其他相关细胞色素C3中发现的完全不同。另一个不寻常的特征是在N末端附近的环中存在由蛋白质提供的氧原子协调的钙离子。据推测，这个环可能因钙离子的存在而稳定，可能有助于血红素氧化还原扰动，甚至可能参与与其氧化还原伙伴识别的特异性。
Crystals of the tetraheme cytochrome c3 from sulfate‐reducing bacteria Desulfovibrio gigas (Dg) (MW 13 kDa, 111 residues, four heme groups) were obtained and X‐ray diffraction data collected to 1.8 Å resolution. The structure was solved by the method of molecular replacement and the resulting model refined to a conventional R‐factor of 14.9%. The three‐dimensional structure shows many similarities to other known crystal structures of tetraheme c3 cytochromes, but it also shows some remarkable differences. In particular, the location of the aromatic residues around the heme groups, which may play a fundamental role in the electron transfer processes of the molecule, are well conserved in the cases of hemes I, III, and IV. However, heme II has an aromatic environment that is completely different to that found in other related cytochromes c3. Another unusual feature is the presence of a Ca2+ ion coordinated by oxygen atoms supplied by the protein within a loop near the N‐terminus. It is speculated that this loop may be stabilized by the presence of this Ca2+ ion, may contribute to heme‐redox perturbation, and might even be involved in the specificity of recognition with its redox partner.
通过二维 1H 核磁共振波谱对脱硫弧菌细胞色素 c3 进行结构研究。
DOI：
10.1042/bj2940909
发表时间：
1993
期刊：
The Biochemical journal
影响因子：
--
作者：
Piçarra-Pereira,MA;Turner,DL;LeGall,J;Xavier,AV
通讯作者：
Xavier,AV
在硫酸盐还原细菌 Desulfovibrio gigas 重建的电子转移链中，从氢中还原亚硫酸盐需要钙。
DOI：
10.1016/s0006-291x(05)81282-3
发表时间：
1991
影响因子：
3.1
作者：
Chen,L;Liu,MY;LeGall,J
通讯作者：
LeGall,J