New qualitative detection methods of genetically modified potatoes.
New qualitative detection methods of genetically modified potatoes.
复制标题
转基因马铃薯定性检测新方法。
DOI：
10.1248/bpb.27.1333
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发表时间：
2004
影响因子：
2
通讯作者：
Misao Kubo
中科院分区：
文献类型：
--
作者：
Takahiro Watanabe;H. Kuribara;T. Mishima;Hiroyuki Kikuchi;T. Kodama;S. Futo;K. Kasama;A. Toyota;Masanori Nouno;A. Saita;Kunihiko Takahashi;A. Hino;H. Akiyama;T. Maitani;Misao Kubo
在日本，8个转基因马铃薯品系(2个新叶马铃薯品系；NL，3个新叶马铃薯品系；NLP，3个新叶Y马铃薯品系；NLY)已被批准在食品和饲料中安全使用。我们建立了对转基因马铃薯进行定性检测的聚合酶链式反应方法，用于NL、NLP和NLY的筛选和鉴定。编码尿苷二磷酸(UDP)-葡萄糖焦磷酸酶(UGPase)的基因被用作分类群特异性基因。我们设计了一对引物来检测CryIIIA基因，作为转基因马铃薯的一种筛选方法，因为该基因应该插入到转基因马铃薯的所有8个品系中。为了鉴定NL、NLP和NLY，我们进一步针对NL、NLP和NLY中不同的重组DNA(r-DNA)设计了三对特异性引物。此外，为了对已导入相同r-DNA的3个NLY品系进行鉴定，设计了3对针对NLY 3号品系r-DNA与基因组DNA边界序列的品系特异性引物。以6个转基因马铃薯品系为供试材料，在相同的聚合酶链式反应条件下，利用每对引物对其进行了特异性鉴定。所有转基因土豆的检出限应在0.1%左右。此外，这些方法的特异性和重复性在六个实验室的合作研究中得到了证实。
In Japan, 8 lines of genetically modified (GM) potato (2 lines of NewLeaf potato; NL, 3 lines of NewLeaf Plus potato; NLP, and 3 lines of NewLeaf Y potato; NLY) have already been authorized as safe for use in foods and feeds. We have developed polymerase chain reaction (PCR) methods for the qualitative detection of the GM potatoes for the screening and the identification of NL, NLP and NLY. The gene encoding uridine diphosphate (UDP)-glucose pyrophosphorylase (UGPase) was used as a taxon specific gene. We designed the primer pair to detect the cryIIIA genes as a screening method for GM potatoes because the gene should be inserted in all 8 lines of the GM potatoes. For identification of NL, NLP and NLY, we further designed three specific primer pairs for the different recombinant DNAs (r-DNA) specifically introduced into NL, NLP, or NLY. In addition, to identify the 3 lines of NLY that have been introduced with the same r-DNA, the three line-specific primer pairs for the border sequence between the r-DNA and genomic DNA of NLY 3 lines were designed. Six lines of GM potato used as the test material were specifically identified using the each primer pair under the same PCR condition. The detection limits of all the GM potatoes should be approximately 0.1%. Furthermore, the specificity and reproducibility of the methods were confirmed in a six-laboratory collaborative study.
使用-seq方法研究癫痫持续状态后早期致痫阶段海马的遗传变化
DOI：
--
发表时间：
2018
期刊：
影响因子：
--
作者：
小澤 千尋;河野 智海;松尾 平;小森 理絵;伊藤 康一
通讯作者：
伊藤 康一
影响因子：
1.5
作者：
Hino, A
通讯作者：
Hino, A