Lack of carbon air filtration impacts early embryo development
Lack of carbon air filtration impacts early embryo development
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DOI：
10.1007/s10815-015-0495-1
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发表时间：
2015-07-01
影响因子：
3.1
通讯作者：
Van Voorhis, Bradley J.
中科院分区：
文献类型：
--
作者：
Munch, Erika M.;Sparks, Amy E.;Van Voorhis, Bradley J.
为了评估在有碳过滤和没有碳过滤的情况下人类受精和着床前胚胎发育，这是一项回顾性队列分析，在单个试管婴儿中心对新鲜的、受控的卵巢过度刺激周期以及先前冷冻保存的原核期胚胎移植周期进行分析。对3组胚胎发育和周期结果进行了比较：1)有碳过滤，2)没有碳过滤，3)恢复碳过滤。共分析了524个新鲜胚胎周期和156个冷冻胚胎周期。新鲜循环的受精率、卵裂率和囊胚转化率均在无碳过滤期间下降，在碳过滤恢复后恢复。在没有过滤的时期解冻和培养的冷冻胚胎与存在碳过滤的时期相比，在卵裂率或囊胚转化率方面没有变化。临床妊娠率和活产率在三个时间段内没有变化。体外受精实验室空气处理机中缺乏碳过滤与新鲜循环中受精不良和早期胚胎发育有关。由于先前冷冻的原核阶段胚胎的发育不受影响，缺乏碳过滤可能优先影响受精期的胚胎。碳过滤是一个成功的人类体外受精实验室不可或缺的一部分。
To assess human fertilization and preimplantation embryo development in the presence and in the absence of carbon filtrationThis is a retrospective cohort analysis of fresh, controlled ovarian hyperstimulation cycles as well as previously cryopreserved pronuclear stage embryo transfer cycles in a single IVF center. Embryo development and cycle-based outcomes were compared among three groups: 1) when carbon filtration was present, 2) when carbon filtration was absent, and 3) when carbon filtration had been restored.A total of 524 fresh cycles and 156 cryopreserved embryo cycles were analyzed. Fertilization, cleavage, and blastocyst conversion rates for fresh cycles all declined during the period of absent carbon filtration and recovered after the restoration of carbon filtration. Cryopreserved embryos that were thawed and cultured during the period of absent filtration did not have changes in cleavage or blastocyst conversion rates compared to periods where carbon filtration was present. Clinical pregnancy and live birth rates were unchanged among the three time periods.The absence of carbon filtration in an IVF laboratory air handler is associated with poor fertilization and early embryo development for fresh cycles. Because development of previously frozen pronuclear stage embryos was unaffected, the lack of carbon filtration may preferentially affect embryos in the peri-fertilization period. Carbon filtration is an integral part to a successful human in-vitro fertilization laboratory.