Three-dimensional cell shapes and arrangements in human sweat glands as revealed by whole-mount immunostaining.
Three-dimensional cell shapes and arrangements in human sweat glands as revealed by whole-mount immunostaining.
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DOI：
10.1371/journal.pone.0178709
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Sekiguchi K
中科院分区：
文献类型：
--
作者：
Kurata R;Futaki S;Nakano I;Fujita F;Tanemura A;Murota H;Katayama I;Okada F;Sekiguchi K
由于汗腺结构的机械收缩促进了汗液的分泌，了解它们的结构-功能关系可以为中暑等汗腺疾病患者提供更有效的治疗方法。传统组织学研究表明，汗腺是由导管和分泌部分组成的三维卷曲管状结构，尽管其详细的结构解剖尚不清楚。为了更好地了解汗腺三维（3D）卷曲结构的细节，采用全贴装染色方法，用汗腺标记物（导管管腔、导管基底、分泌管腔和肌上皮细胞）显示3D卷曲的腺体结构。三维螺旋状腺体结构成像显示导管和分泌部分由不同的管状结构组成。导管小管偶有弯曲，分泌小管常有弯曲，形成自缠绕的盘绕结构。复杂的螺旋状腺体结构的全贴装染色也显示了单个汗腺隔室中详细的3D细胞排列。导管由排列规则的立方细胞组成，而分泌部分被肌上皮细胞包围，沿纠缠的分泌小管纵向伸长。全载染色还用于观察血管和神经纤维的空间排列，它们都有助于汗液的分泌。血管纵向平行于汗腺小管，神经纤维缠绕在分泌小管周围，而不是导管小管。综上所述，汗腺的全载染色揭示了复杂螺旋状腺体结构的3D细胞形状和排列，并提供了对汗液分泌过程中螺旋状腺体结构机械收缩的见解。
Because sweat secretion is facilitated by mechanical contraction of sweat gland structures, understanding their structure-function relationship could lead to more effective treatments for patients with sweat gland disorders such as heat stroke. Conventional histological studies have shown that sweat glands are three-dimensionally coiled tubular structures consisting of ducts and secretory portions, although their detailed structural anatomy remains unclear. To better understand the details of the three-dimensional (3D) coiled structures of sweat glands, a whole-mount staining method was employed to visualize 3D coiled gland structures with sweat gland markers for ductal luminal, ductal basal, secretory luminal, and myoepithelial cells. Imaging the 3D coiled gland structures demonstrated that the ducts and secretory portions were comprised of distinct tubular structures. Ductal tubules were occasionally bent, while secretory tubules were frequently bent and formed a self-entangled coiled structure. Whole-mount staining of complex coiled gland structures also revealed the detailed 3D cellular arrangements in the individual sweat gland compartments. Ducts were composed of regularly arranged cuboidal shaped cells, while secretory portions were surrounded by myoepithelial cells longitudinally elongated along entangled secretory tubules. Whole-mount staining was also used to visualize the spatial arrangement of blood vessels and nerve fibers, both of which facilitate sweat secretion. The blood vessels ran longitudinally parallel to the sweat gland tubules, while nerve fibers wrapped around secretory tubules, but not ductal tubules. Taken together, whole-mount staining of sweat glands revealed the 3D cell shapes and arrangements of complex coiled gland structures and provides insights into the mechanical contraction of coiled gland structures during sweat secretion.