Immunohistochemical Labelling of Cytokines in Calves Infected Experimentally with Mycoplasma bovis
Immunohistochemical Labelling of Cytokines in Calves Infected Experimentally with Mycoplasma bovis
复制标题
DOI：
10.1016/j.jcpa.2015.01.006
复制
发表时间：
2015-02-01
影响因子：
0.8
通讯作者：
Fernandez, A.
中科院分区：
文献类型：
--
作者：
Rodriguez, F.;Castro, P.;Fernandez, A.
为了进一步了解牛支原体相关肺炎的发病机制，检查了不同肺区室中的细胞因子表达。采用免疫组织化学方法检测了10头实验性感染M的小牛肺中肿瘤坏死因子（TNF）-α、白细胞介素（IL）-4和干扰素（IFN）-γ的表达。牛M.牛抗原位于呼吸道上皮细胞和气道腔中的炎性细胞内。TNF-α、IL-4和IFN-γ的免疫标记通常与炎症相关，特别是在增生性支气管相关淋巴组织（BALT）中的巨噬细胞和淋巴细胞中，在增厚的肺泡隔和感染动物的支气管肺泡渗出液中。In M.牛感染、巨噬细胞和淋巴细胞活化导致能够诱导肺损伤和BALT增生的多种细胞因子的表达。检测的细胞因子可能在肺防御M.牛传染病(C)2015爱思唯尔有限公司版权所有。
To gain further insight into the pathogenesis of Mycoplasma bovis-associated pneumonia, cytokine expression in different pulmonary compartments was examined. The expression of tumour necrosis factor (TNF)-alpha, interleukin (IL)-4 and interferon (IFN)-gamma was examined immunohistochemically in the lung of 10 calves infected experimentally with M. bovis. M. bovis antigen was located in respiratory epithelial cells and within inflammatory cells in the airway lumina. Immunolabelling for TNF-alpha, IL-4 and IFN-gamma was usually associated with inflammation, particularly in macrophages and lymphocytes in hyperplastic bronchus-associated lymphoid tissue (BALT), in thickened alveolar septa and in the bronchoalveolar exudate of infected animals. In M. bovis infection, macrophage and lymphocyte activation results in expression of a number of cytokines capable of inducing lung lesions and hyperplasia of the BALT. The cytokines examined likely play a role in pulmonary defence against M. bovis infection. (C) 2015 Elsevier Ltd. All rights reserved.