Limited stability in cell culture medium and hydrogen peroxide formation affect the growth inhibitory properties of delphinidin and its degradation product gallic acid
Limited stability in cell culture medium and hydrogen peroxide formation affect the growth inhibitory properties of delphinidin and its degradation product gallic acid
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DOI：
10.1002/mnfr.200700004
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发表时间：
2007-09-01
影响因子：
5.2
通讯作者：
Marko, Doris
中科院分区：
文献类型：
--
作者：
Kern, Melanie;Fridrich, Diana;Marko, Doris
在本研究中，我们研究了花青素在细胞培养条件下的稳定性，并解决了降解产物是否可能有助于分配给母体化合物的细胞效应的问题。经HPLC/DAD检测，30min后已有明显降解。然而，到目前为止，可检测到的花青素的减少远远超过了各自酚酸的形成。从形成的酚酸中，只有没食子酸(GA)表现出生长抑制特性。然而，也发现GA被迅速降解。此外，与Delphinidin(DEL)或GA孵育导致大量过氧化氢的形成。过氧化氢酶对过氧化氢积累的抑制作用显著改善了DEL和GA的生长抑制作用，提示过氧化氢的形成可能产生实验伪影。综上所述，结果表明，花青素(CY)、天花粉蛋白(PG)、牡丹素(PN)和橙花素(MV)降解形成的酚酸对母体化合物的生长抑制作用没有贡献。DEL的降解产生一种具有显著生长抑制特性(GA)的酚酸。然而，考虑到GA生成的比例较小且缺乏稳定性，GA对DEL生长抑制性能的贡献可能是有限的。
In the present study we investigated the stability of anthocyanidins under cell culture conditions and addressed the question whether degradation products might contribute to the cellular effects assigned to the parent compounds. Substantial degradation was found already after 30 min, measured by HPLC/DAD. However, the decrease of detectable anthocyanidins exceeded by far the formation of the respective phenolic acids. From the formed phenolic acids only gallic acid (GA) exhibited growth inhibitory properties. However, also GA was found to be degraded rapidly. Furthermore, the incubation with delphinidin (DEL) or GA resulted in a substantial formation of hydrogen peroxide. The suppression of hydrogen peroxide accumulation by catalase modified significantly the growth inhibitory effects of DEL and GA, indicating that hydrogen peroxide formation might generate experimental artefacts. In summary, the results show that the phenolic acids formed by the degradation of cyanidin (CY), pelargonidin (PG), peonidin (PN) and malvidin (MV) do not contribute to the growth inhibitory effect of the parent compound. The degradation of DEL generates a phenolic acid with substantial growth inhibitory properties (GA). However, taken into account the small proportion of generated GA and its lacking stability, the contribution of GA to the growth inhibitory properties of DEL might be limited.