Melatonin inhibits seed germination by crosstalk with abscisic acid, gibberellin, and auxin in Arabidopsis
Melatonin inhibits seed germination by crosstalk with abscisic acid, gibberellin, and auxin in Arabidopsis
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褪黑激素通过与拟南芥中脱落酸、赤霉素和生长素的串扰抑制种子发芽
DOI：
10.1111/jpi.12736
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发表时间：
2021-04-20
影响因子：
10.3
通讯作者：
Yu, Diqiu
中科院分区：
文献类型：
--
作者：
Lv, Yan;Pan, Jinjing;Yu, Diqiu
种子萌发是种子植物生活史中的一个重要发育阶段，受复杂的信号调控。褪黑素是一种与种子在胁迫条件下萌发有关的信号分子，尽管其潜在的调控机制在很大程度上尚不清楚。在本研究中，我们发现低浓度的褪黑素(10微米或100微米)对种子萌发没有影响，但当褪黑素浓度增加到500微米或1000微米时，拟南芥种子的萌发受到显著的抑制。RNA测序分析表明，褪黑激素调控种子萌发与植物激素脱落酸(ABA)、赤霉素(GA)和生长素有关。进一步研究发现，ABA和褪黑素对种子萌发有协同抑制作用，而GA和生长素则拮抗褪黑素对种子萌发的抑制作用。褪黑激素生物合成酶基因5-羟色胺N-乙酰转移酶(SNAT)或N-乙酰-5-羟色胺甲基转移酶(ASMT)基因的中断促进种子萌发，而ASMT过表达则抑制种子萌发。综上所述，我们的研究为褪黑素在调节拟南芥种子萌发中的作用和机制提供了新的线索。
Seed germination, an important developmental stage in the life cycle of seed plants, is regulated by complex signals. Melatonin is a signaling molecule associated with seed germination under stressful conditions, although the underlying regulatory mechanisms are largely unknown. In this study, we showed that a low concentration (10 µM or 100 µM) of melatonin had no effect on seed germination, but when the concentration of melatonin increased to 500 µM or 1000 µM, seed germination was significantly inhibited in Arabidopsis. RNA sequencing analysis showed that melatonin regulated seed germination correlated to phytohormones abscisic acid (ABA), gibberellin (GA), and auxin. Further investigation revealed that ABA and melatonin synergistically inhibited seed germination, while GA and auxin antagonized the inhibitory effect of seed germination by melatonin. Disruption of the melatonin biosynthesis enzyme gene serotonin N‐acetyltransferase (SNAT) or N‐acetylserotonin methyltransferase (ASMT) promoted seed germination, while overexpression of ASMT inhibited seed germination. Taken together, our study sheds new light on the function and mechanism of melatonin in modulating seed germination in Arabidopsis.