SOME CHEMICAL PRINCIPLES APPLICABLE TO SOME SILVER AND GOLD STAINING METHODS FOR NEUROPATHOLOGICAL STUDIES
SOME CHEMICAL PRINCIPLES APPLICABLE TO SOME SILVER AND GOLD STAINING METHODS FOR NEUROPATHOLOGICAL STUDIES
复制标题
适用于神经病理学研究的一些银染和金染方法的一些化学原理
DOI：
--
复制
发表时间：
1976
影响因子：
3.2
通讯作者：
I. Feigin
中科院分区：
文献类型：
--
作者：
I. Feigin
结合星形胶质细胞、小胶质细胞、轴突和网状纤维染色方法的经验，分析了神经病理学研究中一些银、金染色方法的一些化学原理。描述了福尔马林固定的作用，以及在分别染色胶质细胞或网状纤维之前用溴化铵或氧化剂处理的作用。银染色方法分为两个根本不同的组。一种方法是使用含或不含其他物质（如碳酸盐或吡啶）的银二胺溶液，在不同的浓度和pH值下，然后进行甲醛还原，有时进行黄金调色。这一组涵盖了各种各样的染色胶质细胞、网状蛋白纤维和轴突的技术。第二种方法是使用银离子的弱溶液，在显影剂中还原，用氯化金处理，再用草酸还原。这一组以染色轴突和网状纤维的方法为代表。对星形胶质细胞的金和氯化汞检测方法作了简要分析。
Some of the chemical principles involved in some silver and gold staining procedures for neuropathological studies are analyzed in the light of experience with methods for astrocytes, microglia, axons and reticulin fibers. The role of formalin fixation, and of treatment with ammonium bromide or oxidizing agents prior to staining for glial cells or reticulin fibers respectively, is described. The silver staining methods are divided into two fundamentally different groups. One is based on the use of silver diammine solutions with or without other substances such as carbonates or pyridine, and at varying concentrations and pH, followed by formaldehyde reduction and, at times, gold toning. This group encompasses a great variety of techniques for staining glial cells, reticulin fibers and axons. The second group is based on the use of weak solutions of silver ions, reduction in a photographic developer, treatment with gold chloride, and a second reduction in oxalic acid. This group is represented by methods for staining axons and reticulin fibers. A brief analysis of the gold and mercuric chloride methods for astrocytes is also given.