Coprophagous features in carnivorous Nepenthes plants: a task for ureases
Coprophagous features in carnivorous Nepenthes plants: a task for ureases
复制标题
肉食性猪笼草植物的粪食特征：脲酶的任务
作者：
Ayufu Yilamujiang;Anting Zhu;R. Ligabue;S. Bartram;C. Witte;R. Hedrich;Mitsuyasu Hasabe;Caroline R. Schöner;Michael G. Schöner;G. Kerth;C. Carlini;A. Mithöfer
大多数陆生食肉植物专门消化昆虫猎物以获得额外的营养。猪笼草属的一些物种与哺乳动物发展了互惠关系，以补充氮。饮食变化是否需要某些酶组合物来利用新的营养来源很少被测试。在这里，我们调查了脲酶的作用，为猪笼草，获得氮从蝙蝠Kerivoula hardwickii，而它栖息在投手。我们假设N. hemsleyana能够利用蝙蝠排泄物中的尿素。事实上，我们证明，15氮富集尿素提供给猪笼草投手代谢和氮分布在植物内。由于尿素酶是降解尿素所必需的，因此应涉及这些水解酶。我们证明了猪笼草植物组织中存在尿素酶及其酶活性。从N.分离并功能性表达Hemsleyana。对包括猪笼草属和其他五种食肉植物分类群在内的真核尿素酶进行了全面的系统发育分析，将它们确定为经典尿素酶并反映了植物系统发育。因此，这项研究揭示了尿素酶作为一个象征性的例子，一个有效的，低成本的，但高适应性的可塑性，在植物中，同时发展一个进一步专业化的生活方式，从食肉动物到食粪。
Most terrestrial carnivorous plants are specialized on insect prey digestion to obtain additional nutrients. Few species of the genus Nepenthes developed mutualistic relationships with mammals for nitrogen supplementation. Whether dietary changes require certain enzymatic composition to utilize new sources of nutrients has rarely been tested. Here, we investigated the role of urease for Nepenthes hemsleyana that gains nitrogen from the bat Kerivoula hardwickii while it roosts inside the pitchers. We hypothesized that N. hemsleyana is able to use urea from the bats’ excrements. In fact, we demonstrate that 15N-enriched urea provided to Nepenthes pitchers is metabolized and its nitrogen is distributed within the plant. As ureases are necessary to degrade urea, these hydrolytic enzymes should be involved. We proved the presence and enzymatic activity of a urease for Nepenthes plant tissues. The corresponding urease cDNA from N. hemsleyana was isolated and functionally expressed. A comprehensive phylogenetic analysis for eukaryotic ureases, including Nepenthes and five other carnivorous plants’ taxa, identified them as canonical ureases and reflects the plant phylogeny. Hence, this study reveals ureases as an emblematic example for an efficient, low-cost but high adaptive plasticity in plants while developing a further specialized lifestyle from carnivory to coprophagy.
影响因子：
7
作者：
Bemm F;Becker D;Larisch C;Kreuzer I;Escalante-Perez M;Schulze WX;Ankenbrand M;Van de Weyer AL;Krol E;Al-Rasheid KA;Mithöfer A;Weber AP;Schultz J;Hedrich R
通讯作者：
Hedrich R