De novo adipose formation in a vascularized engineered construct
De novo adipose formation in a vascularized engineered construct
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DOI：
10.1002/micr.20056
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发表时间：
2004
期刊：
影响因子：
2.1
通讯作者：
R. Walton;E. Beahm;Liza C. Wu
中科院分区：
文献类型：
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作者：
R. Walton;E. Beahm;Liza C. Wu
虽然组织工程领域是一个新兴的领域，但脂肪工程的进展一直滞后，这在很大程度上是由于与体内培养和维持这种独特组织相关的问题。在使用实验大鼠模型的初步研究中，我们在纤维血管支架中使用Matrigel和碱性成纤维细胞生长因子的组合诱导脂肪新生，通过硅胶外壳将构建体与带蒂血液供应（浅表下腹壁血管）隔离，创建工程化三维脂肪组织构建体。对脂肪组织和血管长入进行组织学评估，随后进行连续研究，每4周进行一次，持续16周。我们证明了形状的脂肪组织结构随着时间的推移的持久性，并假设纳入血管供应可能会提高实验诱导的脂肪结构的耐久性，并可能提供一种手段，显微外科转移的结构。© 2004 Wiley利斯公司
While the field of tissue engineering is a burgeoning one, progress with fat engineering has lagged, due in large part to problems associated with nurturing and sustaining this unique tissue in vivo. In a pilot study using an experimental rat model, we induced liponeogenesis with a combination of Matrigel and basic fibroblast growth factor in an fibrovascular scaffold, isolating the construct to a pedicled blood supply (the superficial inferior epigastric vessels) via a silicone housing, creating an engineered three‐dimensional adipose tissue construct. Adipose tissue and vascular ingrowth were assessed histologically and followed by serial study at 4‐week intervals for 16 weeks. We demonstrated persistence of shaped adipose tissue constructs over time, and postulate that incorporating a vascular supply may enhance the durability of experimentally induced fat constructs and potentially provide a means for microsurgical transfer of the construct. © 2004 Wiley‐Liss, Inc.