Collective movement of epithelial cells on a collagen gel substrate
Collective movement of epithelial cells on a collagen gel substrate
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DOI：
10.1529/biophysj.104.047654
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发表时间：
2005-03-01
影响因子：
3.4
通讯作者：
Kawabata, K
中科院分区：
文献类型：
--
作者：
Haga, H;Irahara, C;Kawabata, K
集体细胞运动作为一种有效的策略，在许多生理事件，包括伤口愈合，胚胎发育和形态发生。我们发现，上皮细胞（Madin-Darby犬肾细胞）在胶原凝胶基质上集体沿着一个方向迁移。通过配备孵育系统的相差显微镜捕获在I型胶原凝胶和玻璃基质上培养的Madin-Darby犬肾细胞的延时图像。在凝胶基质上，随着细胞数量的增加，细胞运动的方向逐渐向一个方向聚集，而在玻璃基质上，细胞运动是随机的。我们还观察到“领导”细胞，它延伸出大的层，并伴随着许多“跟随”细胞，在定向胶原纤维的方向迁移。每个细胞运动的均方位移和从细胞速度的空间分布计算的空间相关函数作为观测时间的函数。在凝胶基质的情况下，空间相关长度逐渐增加，代表了多细胞运动的集体性。
Collective cell movement acts as an efficient strategy in many physiological events, including wound healing, embryonic development, and morphogenesis. We found that epithelial cells (Madin-Darby canine kidney cell) migrated collectively along one direction on a collagen gel substrate. Time-lapse images of Madin-Darby canine kidney cells cultured on type-I collagen gels and glass substrates were captured by phase contrast microscopy equipped with an incubation system. On the gel substrate, the directions of cell movement gradually converged on one direction as the number of cells increased, whereas the cells moved randomly on the glass substrate. We also observed "leader'' cells, which extended large lamellae and were accompanied by many "follower'' cells, migrating in the direction of oriented collagen fibers. The mean-squared displacement of each cell movement and the spatial correlation function calculated from the spatial distribution of cell velocity were obtained as functions of observation time. In the case of the gel substrate, the spatial correlation length increased gradually, representing the collectiveness of multicellular movement.