Combined microRNAome and transcriptome analysis of follicular phase and luteal phase in porcine ovaries
Combined microRNAome and transcriptome analysis of follicular phase and luteal phase in porcine ovaries
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猪卵巢卵泡期和黄体期的 microRNAome 和转录组联合分析
DOI：
10.1111/rda.13457
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发表时间：
2019
影响因子：
1.7
通讯作者：
Yin Zongjun
中科院分区：
文献类型：
--
作者：
Zhang Xiaodong;Zhang Liang;Shang Jinnan;Tao Qiangqiang;Tian Mi;Ma Yingchun;Xu Yiliang;Ding Yueyun;Zhou Rong;Li Kui;Yin Zongjun
本研究旨在探讨猪卵泡期（FP）和黄体期（LP）卵巢中miRNAs和mRNAs的表达差异，为哺乳动物生殖调控的研究提供理论依据。使用RNA-Seq和miRNA-Seq鉴定6头母猪（具有相似体重和相同胎次的3岁约克郡猪）卵巢中FP和LP之间的差异表达基因（DEG）和miRNA（DEM）。利用生物信息学方法筛选与猪卵巢功能相关的基因和miRNAs。使用真实的实时定性PCR验证测序结果。RNA-Seq结果显示，与FP相比，LP中有3，078个基因上调，1，444个基因下调，DEG在242个基因本体（GO）术语和33个京都基因和基因组百科全书（KEGG）途径中显著富集。miRNA-Seq鉴定了112个DEM，其中25个在LP中与FP相比上调，87个下调。我们获得了4，522个DEG和从112个DEM预测的2，444个靶基因之间的186个交叉基因（IG）。在构建了miRNA-基因-通路网络后，我们鉴定了关键的miRNA和基因，包括miR-17 - 3 p、miR-214、miR-221 - 5 p、miR-125 b、FGF 1、YWHAG、YWHAZ、FDFT 1和DHCR 24，它们在Hippo和PI 3 K-Akt信号通路以及各种代谢通路中富集。这些结果表明这些关键基因和miRNAs可能在猪卵巢从FP到LP的发育转变中起重要作用，并为进一步研究提供了候选靶点。
The aim of this study was to explore the expression difference of miRNAs and mRNAs between the follicular phase (FP) and luteal phase (LP) in porcine ovaries and provide a theoretical basis for the research on mammalian reproductive regulation. RNA‐Seq and miRNA‐Seq were used to identify differentially expressed genes (DEGs) and miRNAs (DEMs) between the FP and LP in ovaries of six sows (3‐year‐old Yorkshire pigs with similar weights and same parities). Bioinformatic analysis was used to screen potential genes and miRNAs related to porcine ovarian function. Real‐time qualitative PCR was used to validate the sequencing results. RNA‐Seq results showed that 3,078 genes were up‐regulated, and 1,444 genes were down‐regulated in the LP compared with the FP, and DEGs were significantly enriched in 242 Gene Ontology (GO) terms and 33 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. miRNA‐Seq identified 112 DEMs, of which 25 were up‐regulated and 87 were down‐regulated in the LP compared with the FP. We obtained 186 intersection genes (IGs) between the 4,522 DEGs and 2,444 target genes predicted from the 112 DEMs. After constructing a miRNA‐gene‐pathway network, we identified key miRNAs and genes including miR‐17‐3p, miR‐214, miR‐221‐5p, miR‐125b,FGF1,YWHAG,YWHAZ,FDFT1andDHCR24, which are enriched in Hippo and PI3K‐Akt signalling pathways, and various metabolic pathways. These results indicate that these key genes and miRNAs may play important roles in the developmental transition from FP to LP in porcine ovaries and represent candidate targets for further study.