Xenogeneic chondrocytes promote stable subcutaneous chondrogenesis of bone marrow-derived stromal cells.
Xenogeneic chondrocytes promote stable subcutaneous chondrogenesis of bone marrow-derived stromal cells.
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DOI：
10.3892/ijmm.2011.830
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发表时间：
2011-11
影响因子：
5.4
通讯作者：
Ke Xue;Yueqian Zhu;Yingying Zhang;Chengan Chiang;Guangdong Zhou;Kai Liu
中科院分区：
文献类型：
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作者：
Ke Xue;Yueqian Zhu;Yingying Zhang;Chengan Chiang;Guangdong Zhou;Kai Liu
局部微环境可能会改变由骨髓基质细胞（BMSC）分化而来的工程软骨皮下植入后的最终命运。在生长因子或低强度超声引导下进行成软骨分化的BMSC在皮下环境中易于纤维化或血管化，而植入关节软骨缺损中的BMSC可以形成稳定的软骨。我们假设软骨细胞将提供理想的软骨形成环境，从而促进异位软骨细胞表型的维持。为了检验这一假设，我们开发了一种新方法，在异种软骨细胞产生的软骨形成环境中促进 BMSC 发育成软骨细胞，并将异种软骨细胞介导的 BMSC 皮下软骨形成与生长因子产生的皮下软骨形成进行比较。这些结果表明异种软骨细胞引导的BMSCs皮下软骨形成比生长因子诱导的更有效。异种软骨细胞诱导的BMSCs在植入前后培养4周即可形成相对成熟的软骨，缩短了体外诱导时间，皮下植入后维持稳定的软骨表型。
The local microenvironment may change the ultimate fate of engineered cartilage differentiated from bone marrow stromal cells (BMSCs) after subcutaneous implantation. Chondrogenically differentiated BMSCs directed by growth factors or low-intensity ultrasound are apt to fibrose or vascularize in the subcutaneous environment, while BMSCs implanted in articular cartilage defects can form stable cartilage. We hypothesized that chondrocytes would provide an ideal chondrogenic environment, and thus promote the maintenance of the chondrocytic phenotype in ectopia. To test this hypothesis, we developed a new method to promote chondrocyte development from BMSCs in a chondrogenic environment produced by xenogeneic chondrocytes and compared the subcutaneous chondrogenesis of BMSCs mediated by xenogeneic chondrocytes with that produced by growth factors. These results indicate that subcutaneous chondrogenesis of BMSCs directed by xenogeneic chondrocytes is more effective than that induced by growth factors. BMSCs induced by xenogeneic chondrocytes formed relatively mature cartilage before or after implantation, following 4 weeks of culture, which reduced the induction time in vitro and led to maintenance of a stable cartilage phenotype after subcutaneous implantation.