CLIMBING FIBER INDUCED DEPRESSION OF BOTH MOSSY FIBER RESPONSIVENESS AND GLUTAMATE SENSITIVITY OF CEREBELLAR PURKINJE-CELLS
CLIMBING FIBER INDUCED DEPRESSION OF BOTH MOSSY FIBER RESPONSIVENESS AND GLUTAMATE SENSITIVITY OF CEREBELLAR PURKINJE-CELLS
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DOI：
10.1113/jphysiol.1982.sp014103
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发表时间：
1982-01-01
影响因子：
5.5
通讯作者：
TONGROACH, P
中科院分区：
文献类型：
--
作者：
ITO, M;SAKURAI, M;TONGROACH, P
在脱脑的家兔中，从吻侧小叶的细胞外取样细胞。浦肯野细胞通过对侧下橄榄刺激的特征性反应来鉴定。篮子细胞的鉴定是基于没有橄榄反应，也基于它们在与鉴定的浦肯野细胞相邻的分子层上的位置。并对小叶的质量场电位进行了研究。单脉冲刺激同侧或对侧前庭神经，以2/s的速度刺激浦肯野细胞，潜伏期为3-6 ms。这种早期兴奋表现为前庭苔藓纤维、颗粒细胞及其轴突（平行纤维）的激活。类似的早期兴奋也发生在假定的篮状细胞中。以20/s的速度刺激前庭神经，以4/s的速度刺激下橄榄神经，持续25 s/试验，有效地抑制了该神经对浦肯野细胞的早期兴奋，而没有相关的自发放电变化。经济萧条在1997年左右恢复。10分钟。这种恢复之后是持续1小时的缓慢抑制。前庭-橄榄联合刺激在浦肯野细胞反应中没有产生这种抑制。谷氨酸离子电泳应用于浦肯野细胞并结合4/s橄榄细胞刺激抑制了浦肯野细胞的谷氨酸敏感性；天冬氨酸敏感性的下降程度要小得多。抑郁症在1997年左右减轻。10分钟后恢复，随后缓慢下降，持续1小时。只有当谷氨酸敏感性相对较高时才会出现这种下降，这表明微电极正在撞击浦肯野细胞树突。浦肯野细胞对平行纤维的神经递质的突触下化学敏感性参与了前庭神经和下橄榄神经联合刺激后观察到的抑制。
In decerebrate rabbits, cells were sampled extracellularly from the rostral flocculus. Purkinje cells were identified by their characteristic responses to stimulation of the contralateral inferior olive. Identification of basket cells was based on the absence of olivary responses and also on their location in the molecular layer adjacent to identified Purkinje cells. Mass field potentials in the flocculus were also studied. Single pulse stimulation of a vestibular nerve, either ipsilateral or contralateral, at a rate of 2/s excited Purkinje cells with a latency of 3-6 ms. This early excitation represents activation through vestibular mossy fibers, granule cells and their axons (parallel fibers). Similar early excitation also occurred in putative basket cells. Conjunctive stimulation of a vestibular nerve at 20/s and the inferior olive at 4/s, for 25 s/trial, effectively depressed the early excitation of Purkinje cells by that nerve, without an associated change in spontaneous discharge. The depression recovered in .apprx. 10 min. This recovery was followed by the onset of a slow depression lasting for 1 h. Conjunctive vestibular-olivary stimulation produced no such depression in the Purkinje cell responses. Ionophoretic application of glutamate to Purkinje cells in conjunction with 4/s olivary stimulation depressed the glutamate sensitivity of Purkinje cells; aspartate sensitivity was depressed to a much lesser degree. The depression diminished in .apprx. 10 min, but this recovery was succeeded by a slow depression lasting for 1 h. The depression was seen only when glutamate sensitivity was relatively high, suggesting that the micro-electrode was impinging onto Purkinje cell dendrites. Subsynaptic chemosensitivity of Purkinje cells to the putative neurotransmitter of parallel fibers is involved in the depression observed after conjunctive stimulation of a vestibular nerve and the inferior olive.