FoxP2 protein decreases at a specific region in the chick midbrain after hatching
FoxP2 protein decreases at a specific region in the chick midbrain after hatching
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DOI：
10.1016/j.neulet.2023.137119
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发表时间：
2023-02-16
影响因子：
2.5
通讯作者：
Tanaka，Masaki
中科院分区：
文献类型：
--
作者：
Bessho，Chikafusa;Yamada，Shunji;Tanaka，Masaki
Forkhead-box亚类P成员2(FOXP2/FoxP2)蛋白是一种转录因子，调节包括人类语言和动物发声在内的某些大脑功能的发育。虽然FoxP2在脑中的表达与典型的发声习得者斑马雀发声之间的关系已经取得了快速的进展，但包括鸡在内的鸟类发声非习得者的发声之间的关系仍然难以捉摸。由于中脑在先天发声发育中起着关键作用，我们分析了雏鸟中脑中的FoxP2蛋白，它们在孵化前不叫，而在孵化后叫。Western印迹分析显示，与孵化前相比，孵化后雏鸡中脑中FoxP2蛋白的表达显著减少。免疫组织化学定量结果显示，孵化后雏鸡中脑视顶盖灰质纤维层FoxP2免疫反应阳性(Ir)细胞较孵化前明显减少。这些发现支持这样一种观点，即孵化后中脑特定区域的FoxP2-ir细胞数量减少可能与鸟类发声非学习者的先天发声有关。
Forkhead-box subclass P member 2 (FOXP2/FoxP2) protein, a transcription factor, regulates the development of certain brain functions, including human speech and animal vocalization. Although rapid progress has been made in demonstrating a relationship between FoxP2 expression in the brain and vocalization of the zebra finch, a typical vocal learner, the relationship in avian vocal non-learners, including chickens remains elusive. Because the midbrain plays a key role in innate vocalization development, we analyzed the FoxP2 protein in the midbrain of chicks, which do not cheep before hatching but cheep and call after hatching. Western blot analyses showed a significant reduction in FoxP2 protein in the chick midbrain after hatching compared with the findings before hatching. Quantitative immunohistochemistry revealed that FoxP2-immunoreactive (ir) cells significantly decreased at the stratum gris fibrosum (SGFS) of the optic tectum in the chick midbrain after hatching compared with the findings before hatching. These findings support the notion that FoxP2-ir cell numbers decrease at a specific region in the midbrain after hatching may be involved in innate vocalization of avian vocal non-learners.