Changes in gill H+-ATPase and Na+/K+-ATPase expression and activity during freshwater acclimation of Atlantic salmon (Salmo salar)
Changes in gill H+-ATPase and Na+/K+-ATPase expression and activity during freshwater acclimation of Atlantic salmon (Salmo salar)
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DOI：
10.1242/jeb.050633
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发表时间：
2011-07-01
影响因子：
2.8
通讯作者：
Schulte, Patricia M.
中科院分区：
文献类型：
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作者：
Bystriansky, Jason S.;Schulte, Patricia M.
很少有研究研究鲑鱼鳃离子转运蛋白在从海水到淡水的过渡过程中表达的变化，这是鲑鱼生命周期的关键时刻。将适应海水的大西洋鲑鱼转移到淡水中，并在 30 天的适应过程中对血液和鳃组织进行取样。在整个实验过程中，海水中的鲑鱼具有稳定的血浆渗透压以及钠和氯化物水平。接触淡水后，血浆钠和氯化物水平以及总渗透压显着下降，然后随着时间的推移恢复到控制水平。接触淡水14天后，鳃H+-ATP酶活性增加了45%以上，而H+-ATP酶mRNA水平不受盐度变化的影响。在暴露于淡水的 4 天内，鳃 Na+/K+-ATP 酶活性比对照水平增加了 43%，直到 30 天采样组下降回到对照水平之前仍显着较高。这种活性的增加与 Na+/K+-ATP 酶同种型 α 1a mRNA 水平增加超过 7 倍相关，并且与 Na+/K+-ATP 酶同种型 α 1b mRNA 水平减少 60% 相关。 Na+/K+-ATPase 亚型 α 1c 和 α 3 的 mRNA 水平并未因淡水暴露而发生变化。小膜蛋白 FXYD 11 和 β 1 亚基的 mRNA 表达的时间进程非常相似，接触淡水后 7 天水平显着增加，然后在 30 天时回落至对照水平。总而言之，这些数据表明 Na+/K+-ATP 酶在大西洋鲑鱼的淡水驯化中发挥着重要作用。
Few studies have examined changes in salmon gill ion transporter expression during the transition from seawater to freshwater, a pivotal moment in the salmonid life cycle. Seawater-acclimated Atlantic salmon were transferred to freshwater and blood and gill tissue were sampled over 30 days of acclimation. Salmon held in seawater had stable plasma osmolality and sodium and chloride levels throughout the experiment. Following freshwater exposure, plasma sodium and chloride levels and total osmolality decreased significantly before returning towards control levels over time. Gill H+-ATPase activity increased by more than 45% 14 days after exposure to freshwater, whereas H+-ATPase mRNA levels were not affected by the salinity change. Within 4 days of freshwater exposure, gill Na+/K+-ATPase activity increased similar to 43% over control levels, remaining significantly higher until the 30 day sampling group when it declined back to control levels. This increase in activity was associated with a more than 7-fold increase in Na+/K+-ATPase isoform alpha 1a mRNA level and a similar to 60% decrease in Na+/K+-ATPase isoform alpha 1b mRNA level. The mRNA levels of Na+/K+-ATPase isoforms alpha 1c and alpha 3 did not change as a result of freshwater exposure. The time courses for mRNA expression of the small membrane protein FXYD 11 and the beta 1-subunit were very similar, with levels increasing significantly 7 days following freshwater exposure before subsiding back to control levels at 30 days. Taken together, these data suggest an important role for Na+/K+-ATPase in freshwater acclimation in Atlantic salmon.