Early-age heat exposure affects skeletal muscle satellite cell proliferation and differentiation in chicks
Early-age heat exposure affects skeletal muscle satellite cell proliferation and differentiation in chicks
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DOI：
10.1152/ajpregu.2001.281.1.r302
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发表时间：
2001-07-01
影响因子：
2.8
通讯作者：
Yahav, S
中科院分区：
文献类型：
--
作者：
Halevy, O;Krispin, A;Yahav, S
将雏鸡暴露于热调节（TC;即，37 ℃ 24小时）导致在以后的年龄显着改善身体和肌肉生长。我们假设TC导致卫星细胞增殖增加，这是进一步肌肉肥大所必需的。立即增加卫星细胞DNA合成中观察到的文化和在体内的TC的3日龄雏鸡的水平显着高于对照组的鸡。这是伴随着一个显着的诱导胰岛素样生长因子-I（IFG-I），但不是肝细胞生长因子在胸肌。未观察到治疗组间血浆IGF-I水平的显著差异。肌肉调节因子在第5天显著升高，随后细胞增殖在第6天下降，TC鸡肌肉中IGF-I持续高水平，这可能表明细胞分化加速。这些数据表明，IGF-I在热暴露后立即刺激卫星细胞肌源性过程中起着重要作用。
Exposure of young chicks to thermal conditioning (TC; i.e., 37 degreesC for 24 h) resulted in significantly improved body and muscle growth at a later age. We hypothesized that TC causes an increase in satellite cell proliferation, necessary for further muscle hypertrophy. An immediate increase was observed in satellite cell DNA synthesis in culture and in vivo in response to TC of 3-day-old chicks to levels that were significantly higher than those of control chicks. This was accompanied by a marked induction of insulin-like growth factor-I (IFG-I), but not hepatocyte growth factor in the breast muscle. No significant difference between treatments in plasma IGF-I levels was observed. A marked elevation in muscle regulatory factors on day 5, followed by a decline in cell proliferation on day 6 together with continuous high levels of IGF-I in the TC chick muscle may indicate accelerated cell differentiation. These data suggest a central role for IGF-I in the immediate stimulation of satellite cell myogenic processes in response to heat exposure.