Hypoglycemic Effect of the Water Extract of Pu-erh Tea
Hypoglycemic Effect of the Water Extract of Pu-erh Tea
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DOI：
10.1021/jf302426w
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发表时间：
2012-10-10
影响因子：
6.1
通讯作者：
Zou, Xiao-Qing
中科院分区：
文献类型：
--
作者：
Du, Wan-hong;Peng, Sheng-Ming;Zou, Xiao-Qing
本文报道了普洱茶水提物的提取和组成，以及普洱茶水提物在体内和体外的降糖作用，探讨了普洱茶水提物对糖尿病的降糖作用。采用高效液相色谱法和比色法对茶中儿茶素、咖啡因、多酚、氨基酸和多糖进行了分析。在体外实验中，测定了wid对HepG2细胞葡萄糖摄取的影响，以及对大鼠肠道蔗糖酶、麦芽糖酶和猪胰腺淀粉酶的抑制作用。然后，采用2型糖尿病患者db/db （BKS）进行空腹和口服葡萄糖耐量试验（OGTT），胃内给药组的血糖和胰岛素水平。g-m +/+ Lepr(db)/J)小鼠体内测定。结果表明，黄芪多糖具有剂量依赖性，显著增加HepG2细胞对葡萄糖的摄取，抑制大鼠肠道蔗糖酶、麦芽糖酶和猪胰腺淀粉酶活性。给药4周后，可抑制过夜禁食小鼠血液中胰岛素和葡萄糖水平的升高。在OGTT中，在db/db小鼠中，哭泣改善了受损的葡萄糖耐量，改善了60和120分钟的胰岛素反应迟缓。这些结果表明，哭泣对2型糖尿病的葡萄糖稳态和胰岛素抵抗的改善有有益的作用。
The water extraction and composition of pu-erh tea, as well as the hypoglycemic effect of the water extract of pu-erh tea (WEPT) in vivo and in vitro, are reported to investigate its hypoglycemic effect on diabetes. High-performance liquid chromatography and colorimetric methods are used to analyze the tea catechins, caffeine, polyphenols, amino acids, and polysaccharides of the WEPT. The effect of the WEPT on glucose uptake by cultured HepG2 cells and the inhibition effect of rat intestinal sucrase, maltase, and porcine pancreatic amylase are determined in vitro. Then, the blood glucose and insulin levels of intragastrically administered WEPT on fasting and oral glucose tolerance test (OGTT) using type 2 diabetic db/db (BKS.Cg-m +/+ Lepr(db)/J) mice are determined in vivo. The results showed that the WEPT dose-dependently and significantly increased glucose uptake by HepG2 cells and inhibited rat intestinal sucrase, maltase, and porcine pancreatic amylase activity. The WEPT intragastrically given for 4 weeks suppressed the increase in blood insulin and glucose levels of db/db mice fasted overnight. In OGTT, the WEPT improved impaired glucose tolerance and ameliorated retarded insulin response at 60 and 120 min in db/db mice. These results suggest that the WEPT has beneficial effects on glucose homeostasis in type 2 diabetes and in amendment of insulin resistance.