Construction of a thermostable cell adhesion protein for reverse transfection.
Construction of a thermostable cell adhesion protein for reverse transfection.
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DOI：
10.1016/j.jbiotec.2010.09.960
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发表时间：
2010-11
影响因子：
4.1
通讯作者：
JooYoun Bae;Sayaka Goto;M. Mie;E. Kobatake
中科院分区：
文献类型：
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作者：
JooYoun Bae;Sayaka Goto;M. Mie;E. Kobatake
转染阵列可用于一次分析多个基因。为了进行基因转染，将细胞培养在板上，在板上标记基因以形成细胞外基质。然而，这种方法受到细胞粘附性和转染效率低的限制。为了克服这些问题，我们试图构建一种由多种功能肽组成的新型细胞外基质蛋白。我们将弹性蛋白衍生的肽Ala-Pro-Gly-Val-Gly-Val （APGVGV）与细胞粘附肽Pro-His-Ser-Arg-Asn （PHSRN）和Arg-Gly-Asp （RGD）融合。得到的融合蛋白E12PSGR具有较高的细胞粘附活性、转染效率和热稳定性。
Transfection arrays are useful to analyze multiple genes at one time. In order to carry out gene transfection, cells are cultured on a plate on which genes are spotted to make extracellular matrix. However, this method is limited by low cell adhesion and transfection efficiency. To overcome these problems, we attempted to construct a novel extracellular matrix protein consisting of a variety of functional peptides. Here we fused the elastin derived peptide Ala-Pro-Gly-Val-Gly-Val (APGVGV) with the cell adhesive peptides, Pro-His-Ser-Arg-Asn (PHSRN) and Arg-Gly-Asp (RGD). The resulting fusion proteins, E12PSGR, had high cell adhesive activity, transfection efficiency, and thermal stability.