Graphene oxide-based injectable conductive hydrogel dressing with immunomodulatory for chronic infected diabetic wounds
Graphene oxide-based injectable conductive hydrogel dressing with immunomodulatory for chronic infected diabetic wounds
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具有免疫调节作用的基于氧化石墨烯的可注射导电水凝胶敷料，用于治疗慢性感染的糖尿病伤口
DOI：
10.1016/j.matdes.2022.111284
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Wenrui Qu
中科院分区：
文献类型：
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作者：
Xiaolan Ou;Lin Guan;Wenlai Guo;Xi Zhang;Siyu Wu;Deming Guo;Ruiyan Li;Andrei V. Zvyagin;Quan Lin;Wenrui Qu
以糖尿病伤口为代表的慢性伤口的主要挑战是感染不易控制，愈合困难，主要是由于高糖微环境、M1巨噬细胞聚集和持续炎症。先前受内源性电场启发的研究已经证明，导电材料可以促进细胞增殖、迁移等，促进伤口愈合然而，还没有研究导电材料对局部免疫的影响。为此，本研究设计了一种基于氧化石墨烯的导电水凝胶来修复感染的糖尿病伤口。以氧化透明质酸、N-羧乙基壳聚糖、氧化石墨烯和多粘菌素B为原料，通过动态席夫碱反应和静电相互作用合成了可注射自愈合导电水凝胶。研究发现该水凝胶具有良好的生物相容性和抗菌性能。此外，体内实验证实，该水凝胶可调节巨噬细胞极化以改善局部炎症微环境，促进新生血管形成，并显著加速糖尿病伤口的愈合。最值得注意的是，基于氧化石墨烯的导电水凝胶可以调节糖尿病伤口中的局部免疫力以促进愈合。这项研究为扩大导电材料的研究提供了新的指导方针，并为促进感染的糖尿病伤口愈合提供了新的方向。
The main challenges of chronic wounds represented by diabetic wounds are that the infection is not easily controlled and the healing is difficult, mainly due to the high-glucose microenvironment, M1 macrophage aggregation, and persistent inflammation. Previous studies inspired by endogenous electric fields have demonstrated that conductive materials can promote cell proliferation, migration, etc., to promote wound healing. However, no study has been conducted on the effect of conductive materials on local immunity. To this end, this study designed a graphene oxide-based conductive hydrogel to repair infected diabetic wounds. Self-healing injectable conductive hydrogels were synthesized by dynamic Schiff-base reaction and electrostatic interactions between oxidized hyaluronic acid,N-carboxyethyl chitosan, graphene oxide, and polymyxin B. The study found that the hydrogel has good biocompatibility and antibacterial properties. In addition, in vivo experiments confirmed that the hydrogel could modulate macrophage polarization to improve the local inflammatory microenvironment, promote neovascularization, and significantly accelerate the healing of diabetic wounds. Most notably, graphene oxide-based conductive hydrogels can modulate local immunity in diabetic wounds to promote healing. This study provides new guidelines for expanding research on conductive materials and new directions for promoting healing in infected diabetic wounds.