Inhibiting microbial adhesion to denture base acrylic resin by titanium dioxide coating
Inhibiting microbial adhesion to denture base acrylic resin by titanium dioxide coating
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DOI：
10.1111/j.1365-2842.2009.02012.x
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发表时间：
2009-12-01
影响因子：
2.9
通讯作者：
Sakurai, K.
中科院分区：
文献类型：
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作者：
Arai, T.;Ueda, T.;Sakurai, K.
P>；义齿的机械清洁在预防吸入性肺炎和假牙口炎等感染方面是有效的。然而，对于身体残疾的假牙佩戴者和老年人来说，机械清洁可能会带来问题。本研究的目的是探讨义齿基托树脂表面涂覆二氧化钛(TiO2)对口腔微生物黏附的抑制作用。制备了大小均匀(10 mm×10 mm×0中心点5 mm)的丙烯酸树脂板材，将其分为两组(未涂覆组和二氧化钛包覆组)。将培养皿浸泡在培养的血链球菌或白色念珠菌中培养24 h，孵育后冲洗去除松散附着的微生物，再培养24 h，用含有苯扎溴铵的试剂检测微生物的三磷酸腺苷(ATP)含量，提取细胞内三磷酸腺苷。此外，为了确定生物膜的形成，我们还对每个平板进行了扫描电子显微镜(SEM)观察。我们发现，无论是血链球菌还是白色念珠菌，其ATP含量都被TiO(2)包覆(P=0，中心点，000)所降低。扫描电子显微镜观察证实，二氧化钛涂层抑制了生物膜的形成。结果表明，在义齿基托树脂表面涂覆一层二氧化钛对血链球菌和白色念珠菌的黏附有抑制作用。
P>Mechanical cleaning of dentures is effective in preventing infections such as aspiration pneumonia and denture stomatitis. For denture wearers with a physical handicap and the elderly, however, mechanical cleaning can present problems. The aim of this study was to investigate the effect of coating denture base acrylic resin with titanium dioxide (TiO(2)) in the inhibition of oral microbial adhesion. We prepared uniformly sized acrylic resin plates (10 mm x 10 mm x 0 center dot 5 mm), which were divided into two groups (a non-coated group and a TiO(2)-coated group). The plates were immersed in cultured Streptococcus sanguinis or Candida albicans and incubated for 24 h. After incubation, each plate was washed to remove loosely adherent microorganisms, and then incubated for a further 24 h. Adenosine triphosphate (ATP) content of the microorganisms was evaluated using a reagent containing benzalkonium, which extracts intra-cellular ATP. In addition, to determine biofilm formation, we also observed each plate by scanning electron microscopy (SEM). We found that the ATP content of both S. sanguinis and C. albicans was reduced by the TiO(2) coating (P = 0 center dot 000). Observation by SEM confirmed that the TiO(2) coating inhibited biofilm formation. The results indicate that a TiO(2) coating on a denture base acrylic resin inhibits adhesion of S. sanguinis and C. albicans.