Bacterial and Plant Signal Integration via D3-Type Cyclins Enhances Symptom Development in the Arabidopsis-Rhodococcus fascians Interaction1[C][W]
Bacterial and Plant Signal Integration via D3-Type Cyclins Enhances Symptom Development in the Arabidopsis-Rhodococcus fascians Interaction1[C][W]
复制标题
通过 D3 型细胞周期蛋白整合细菌和植物信号增强拟南芥-红球菌筋膜相互作用中的症状发展1[C][W]
作者：
Elisabeth Stes;S. Biondi;M. Holsters;D. Vereecke
The phytopathogenic actinomycete Rhodococcus fascians drives its host to form a nutrient-rich niche by secreting a mixture of cytokinins that triggers plant cell division and shoot formation. The discrepancy between the relatively low amount of secreted cytokinins and the severe impact of R. fascians infection on plant development has puzzled researchers for a long time. Polyamine and transcript profiling of wild-type and cytokinin receptor mutant plants revealed that the bacterial cytokinins directly stimulated the biosynthesis of plant putrescine by activating arginine decarboxylase expression. Pharmacological experiments showed that the increased levels of putrescine contributed to the severity of the symptoms. Thus, putrescine functions as a secondary signal that impinges on the cytokinin-activated pathway, amplifying the hormone-induced changes that lead to the formation of a leafy gall. Exogenous putrescine and treatment with polyamine biosynthesis inhibitors combined with transcript and polyamine analyses of wild-type and mutant plants indicated that the direct target of both the bacterial cytokinins and plant putrescine was the expression of D3-type cyclins. Hence, the activated d-type cyclin/retinoblastoma/E2F transcription factor pathway integrates both external and internal hormonal signals, stimulating mitotic cell divisions and inducing pathological plant organogenesis.
DOI：
10.1073/pnas.0704166104
发表时间：
2007-09-04
影响因子：
11.1
作者：
Dewitte, Walter;Scofield, Simon;Murray, James A. H.
通讯作者：
Murray, James A. H.