Heparan sulfate proteoglycan synthesis and its expression are decreased in the retina of diabetic rats
Heparan sulfate proteoglycan synthesis and its expression are decreased in the retina of diabetic rats
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DOI：
10.1076/ceyr.16.2.127.5089
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发表时间：
1997-02-01
影响因子：
2
通讯作者：
Reddi, AS
中科院分区：
文献类型：
--
作者：
Bollineni, JS;Alluru, I;Reddi, AS
目的.硫酸乙酰肝素蛋白聚糖（HSPG）是所有基底膜的组成部分，并与这些基底膜的电荷选择性特性有关。研究表明，糖尿病动物视网膜毛细血管和肾小球基底膜中HSPG阴离子位点的数量减少。本研究检测了链脲佐菌素糖尿病大鼠视网膜中HSPG合成和其作为串珠素表达的减少是否减少。通过腹腔注射链脲佐菌素诱导雄性Wistar大鼠糖尿病，20周后处死大鼠。分离视网膜，并通过将S-35-硫酸盐掺入硫酸乙酰肝素中来评估HSPG合成。通过RNA酶保护试验定量串珠素mRNA的表达。糖尿病大鼠视网膜HSPG合成及串珠素mRNA表达均较正常大鼠减少。HSPG合成的减少可能是糖尿病患者视网膜基底膜阴离子位点减少和毛细血管通透性增加的原因。
Purpose. Heparan sulfate proteoglycan (HSPG) is an integral component of all basement membranes and is implicated in the charge-selective properties of these basement membranes. Studies have shown a decrease in the number of HSPG anionic sites in the retinal capillary and glomerular basement membrane of diabetic animals. This study examined whether a decrease in both HSPG synthesis and its expression as perlecan are reduced in the retina of streptozotocin-diabetic rats.Methods. Diabetes was induced in male Wistar rats by intraperitoneal injection of streptozotocin and the rats were sacrificed 20 weeks later. Retinas were isolated and HSPG synthesis was assessed by incorporation of S-35-sulfate into heparan sulfate. The expression of mRNA for perlecan was quantified by RNase protection assay.Results. Both the synthesis of HSPG and mRNA expression for perlecan were decreased in the retina of diabetic compared to normal rats.Conclusions. The decrease in HSPG synthesis may account for the reported decrease in retinal basement membrane anionic sites and increased capillary permeability that occurs in diabetes.