Temporal alterations in cardiac fibroblast function following induction of pressure overload.
Temporal alterations in cardiac fibroblast function following induction of pressure overload.
复制标题
DOI：
10.1007/s00441-010-0943-2
复制
发表时间：
2010-04
影响因子：
3.6
通讯作者：
Carver, Wayne
中科院分区：
文献类型：
--
作者：
Stewart, James A., Jr.;Massey, Erin P.;Fix, Charity;Zhu, Jinyu;Goldsmith, Edie C.;Carver, Wayne
已知心血管负荷增加（压力超负荷）会引起心室重塑，包括心肌细胞肥大和间质纤维化。虽然许多研究都集中在心肌细胞肥大的机制，相对较少的是已知的间质成纤维细胞的反应，增加心血管负荷。成纤维细胞是哺乳动物心肌中数量最多的细胞类型，长期以来被认为是产生大部分心肌细胞外基质的细胞。现在才意识到成纤维细胞行为的其他方面对整体心脏功能很重要。本研究旨在研究心血管负荷增加时成纤维细胞活性的时间变化。在腹主动脉缩窄诱导压力超负荷后的特定时间点（3、7、14和28天）分离大鼠心肌成纤维细胞。进行生物测定以测量成纤维细胞功能的特定参数，包括三维胶原凝胶的重塑和收缩、迁移和增殖。此外，还检测了整合素家族的细胞外基质受体的表达。腹主动脉缩窄后7天内心肌肥厚和纤维化明显。胶原凝胶收缩，迁移和增殖增强成纤维细胞从压力超载的动物相比，从假手术动物的成纤维细胞。成纤维细胞功能和蛋白质表达的差异在主动脉缩窄7天内是明显的，同时伴随着完整心肌的肥大和纤维化的发生。这些数据进一步支持了成纤维细胞表型的快速和动态变化伴随并促进心血管疾病进展的观点。
Increases in cardiovascular load (pressure overload) are known to elicit ventricular remodeling including cardiomyocyte hypertrophy and interstitial fibrosis. While numerous studies have focused on the mechanisms of myocyte hypertrophy, comparatively little is known regarding the response of the interstitial fibroblasts to increased cardiovascular load. Fibroblasts are the most numerous cell type in the mammalian myocardium and have long been recognized as producing the majority of the myocardial extracellular matrix. It is only now becoming appreciated that other aspects of fibroblast behavior are important to overall cardiac function. The present studies were performed to examine the temporal alterations in fibroblast activity in response to increased cardiovascular load. Rat myocardial fibroblasts were isolated at specific time-points (3, 7, 14, and 28 days) after induction of pressure overload by abdominal aortic constriction. Bioassays were performed to measure specific parameters of fibroblast function including remodeling and contraction of 3-dimensional collagen gels, migration, and proliferation. In addition, the expression of extracellular matrix receptors of the integrin family was examined. Myocardial hypertrophy and fibrosis were evident within 7 days after constriction of the abdominal aorta. Collagen gel contraction, migration, and proliferation were enhanced in fibroblasts from pressure-overloaded animals compared to fibroblasts from sham animals. Differences in fibroblast function and protein expression were evident within 7 days of aortic constriction, concurrent with the onset of hypertrophy and fibrosis of the intact myocardium. These data provide further support for the idea that rapid and dynamic changes in fibroblast phenotype accompany and contribute to the progression of cardiovascular disease.
登录
查看更多内容
影响因子：
5
作者：
Flack, EC;Lindsey, ML;Spinale, FG
通讯作者：
Spinale, FG
DOI：
10.1152/ajpheart.00514.2007
发表时间：
2007-09-01
影响因子：
4.8
作者：
Banerjee, Indroneal;Fuseler, John W.;Baudino, Troy A.
通讯作者：
Baudino, Troy A.
影响因子：
20.1
作者：
BURGESS, ML;CARVER, WE;BORG, TK
通讯作者：
BORG, TK
影响因子：
10.8
作者：
Marganski, WA;De Biase, VM;Dembo, M
通讯作者：
Dembo, M
DOI：
10.1111/j.1749-6632.1995.tb17446.x
发表时间：
1995-01-01
期刊：
CARDIAC GROWTH AND REGENERATION
影响因子：
--
作者：
BUTT, RP;LAURENT, GJ;BISHOP, JE
通讯作者：
BISHOP, JE