Low aspect ratio micropores for single-particle and single-cell analysis
Low aspect ratio micropores for single-particle and single-cell analysis
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DOI：
10.1002/elps.201400570
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发表时间：
2015-05-01
期刊：
影响因子：
2.9
通讯作者：
Kim, Min Jun
中科院分区：
文献类型：
--
作者：
Goyal, Gaurav;Mulero, Rafael;Kim, Min Jun
本文介绍了微粒和细菌易位的研究，使用低纵横比的固态微孔。在200纳米厚的自支撑氮化硅膜中制造了直径5 m的微孔，从而产生了具有极低纵横比（名义上为0.04）的孔。对于微粒易位实验，使用尺寸范围为1-4 μ m的磺化聚苯乙烯微粒和磁性微珠。使用微粒易位的特点，我们发现，颗粒易位的结果，在孔的几何电阻的变化，而访问电阻保持不变。此外，我们证明了我们的探测器的能力，探测高分辨率的形状信息的易位分析物使用串联磁性微球。对于多珠体系结构的每个微球观察到不同的电流降峰。对于细菌易位实验，使用无鞭毛大肠杆菌（菌株HCB 5）和野生型有鞭毛鼠伤寒沙门氏菌（菌株SJW 1103）。获得了两种细菌的不同电流特征，这种易位行为的差异归因于细菌上不同的表面蛋白分布。我们的研究结果可能有助于开发低纵横比孔，用于高分辨率微粒表征和单细胞分析。
This paper describes microparticle and bacterial translocation studies using low aspect ratio solid-state micropores. Micropores, 5 m in diameter, were fabricated in 200 nm thick free-standing silicon nitride membranes, resulting in pores with an extremely low aspect ratio, nominally 0.04. For microparticle translocation experiments, sulfonated polystyrene microparticles and magnetic microbeads in size range of 1-4 m were used. Using the microparticle translocation characteristics, we find that particle translocations result in a change only in the pore's geometrical resistance while the access resistance remains constant. Furthermore, we demonstrate the ability of our micropore to probe high-resolution shape information of translocating analytes using concatenated magnetic microspheres. Distinct current drop peaks were observed for each microsphere of the multibead architecture. For bacterial translocation experiments, nonflagellated Escherichia coli (strain HCB 5) and wild type flagellated Salmonella typhimurium (strain SJW1103) were used. Distinct current signatures for the two bacteria were obtained and this difference in translocation behavior was attributed to different surface protein distributions on the bacteria. Our findings may help in developing low aspect ratio pores for high-resolution microparticle characterization and single-cell analysis.