EFFECT OF STRENUOUS EXERCISE ON PLATELET ACTIVATION STATE AND REACTIVITY
EFFECT OF STRENUOUS EXERCISE ON PLATELET ACTIVATION STATE AND REACTIVITY
复制标题
DOI：
10.1161/01.cir.88.4.1502
复制
发表时间：
1993-10-01
期刊：
影响因子：
37.8
通讯作者：
MICHELSON, AD
中科院分区：
文献类型：
--
作者：
KESTIN, AS;ELLIS, PA;MICHELSON, AD
背景据推测，血小板被激活，或更活跃，剧烈运动，这些变化可能在运动诱导的冠状动脉缺血的发生中发挥作用。以前的研究产生了相互矛盾的结果，但使用的测定（例如，血小板聚集，血浆血小板因子4和血浆β-血小板球蛋白）受到方法学问题的影响。在本研究中，全血流式细胞术方法被用来研究血小板活化状态和反应性的12个体力活动和12个久坐的个人之前和之后的标准化平板运动试验。本试验中包括肽gly-pro-arg-pro（GPRP），以防止纤维蛋白聚合和血小板聚集，从而允许测量全血生理环境中单个血小板对凝血酶的反应性。一组荧光标记的单克隆抗体用于监测活化依赖性血小板表面变化：糖蛋白（GP）Ib（6D 1）下调和GMP-140（S12）、GPIIb-IIIa复合物（PAC 1）和GPIV（OKMS）上调。在运动前获得的样本中，未暴露于凝血酶的血小板未显示出体外活化的证据。在久坐的受试者中，运动引起血小板活化状态和反应性的一致和显著增强，这通过凝血酶0.05 U/mL存在时6D 1的结合来判断（P
Background. It has been hypothesized that platelets are activated, or made more activatible, by strenuous exercise and that these changes may play a role in the genesis of exercise-induced coronary ischemia. Previous studies have yielded conflicting results but have used assays (eg, platelet aggregation, plasma platelet factor 4, and plasma beta-thromboglobulin) that are subject to methodological problems.Methods and Results. In the present study, a whole blood flow cytometric method was used to study the platelet activation state and reactivity of 12 physically active and 12 sedentary individuals before and after standardized treadmill exercise testing. The peptide gly-pro-arg-pro (GPRP) was included in this assay to prevent fibrin polymerization and platelet aggregation, thus allowing the measurement of the reactivity to thrombin of individual platelets in the physiological milieu of whole blood. A panel of fluorescent-labeled monoclonal antibodies was used to monitor activation-dependent platelet surface changes: downregulation of glycoprotein (GP) Ib (6D1) and upregulation of GMP-140 (S12), the GPIIb-IIIa complex (PAC1), and GPIV (OKMS). In samples obtained before exercise, platelets not exposed to thrombin showed no evidence of in vitro activation. In the sedentary subjects, exercise caused a consistent and significant augmentation of the platelet activation state and reactivity as judged by the binding of 6D1 in the presence of thrombin 0.05 U/mL (P