Suppression of lymphocyte activation by a high-molecular-weight glycoprotein released from preimplantation ovine and porcine conceptuses.
Suppression of lymphocyte activation by a high-molecular-weight glycoprotein released from preimplantation ovine and porcine conceptuses.
复制标题
植入前绵羊和猪胚胎释放的高分子量糖蛋白抑制淋巴细胞活化。
DOI：
10.1111/j.1600-0897.1987.tb00115.x
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Roberts,RM
中科院分区：
文献类型：
--
作者：
Murray,MK;Segerson,EC;Hansen,PJ;Bazer,FW;Roberts,RM
从着床前、延长期绵羊（第16天和第17天）和猪（第16天）孕体的孵育培养基中纯化了一种高分子量（MW > 660，000）酸性糖蛋白（HMWGP）。检测了HMWGP抑制[3 H-甲基]胸苷掺入植物血凝素或双向混合淋巴细胞培养物刺激的淋巴细胞的能力。绵羊和猪HMWGP以剂量依赖性方式抑制[3 H-甲基]胸苷掺入淋巴细胞。在最低试验剂量下检测到约50%的抑制（绵羊，25 μg/ml;猪，5 μg/ml）。在评价的最高剂量下（绵羊，200 μg/ml;猪，40 μg/ml），胸苷掺入完全抑制。这种免疫抑制作用不是通过台盼蓝拒染法评价的对淋巴细胞的总体细胞毒性作用的结果。总之，绵羊和猪孕体糖蛋白，HMWGP，在植物血凝素和混合淋巴细胞培养物中具有有效的体外免疫抑制活性。在体内，HMWGP可能在绵羊和猪的妊娠早期具有免疫调节作用，通过在子宫内提供局部免疫抑制环境来防止孕体排斥。
A high‐molecular‐weight (MW > 660,000), acidic glycoprotein (HMWGP) was purified from incubation medium of preimplantation, elongating ovine (day 16 and 17) and porcine (day 16) conceptuses. HMWGP was tested for its ability to inhibit [3H‐methyl]thymidine incorporation into lymphocytes stimulated by phytohemagglutinin or two‐way mixed lymphocyte cultures. Ovine and porcine HMWGP inhibited the incorporation of [3H‐methyl]thymidine into lymphocytes in a dose‐dependent manner. An approximately 50% inhibition was detected at the lowest dose tested (ovine, 25 μg/ml; porcine, 5 μg/ml). Complete suppression of thymidine incorporation occurred at the highest doses evaluated (ovine, 200 μg/ml; porcine, 40 μg/ml). This immunosuppressive effect was not the result of an overall cytotoxic effect on lymphocytes as evaluated by trypan blue exclusion. In conclusion, an ovine and porcine conceptus glycoprotein, HMWGP, has potent in vitro immunosuppressive activity in both phytohemagglutinin and mixed lymphocyte cultures. In vivo, HMWGP may have an immunoregulatory role during early pregnancy in the sheep and pig by providing a local immunosuppressive environment within the uterus to prevent conceptus rejection.