In vitro generation of lymphohematopoietic cells from endothelial cells purified from murine embryos
In vitro generation of lymphohematopoietic cells from endothelial cells purified from murine embryos
复制标题
DOI：
10.1016/s1074-7613(00)80581-6
复制
发表时间：
1998-06-01
期刊：
影响因子：
32.4
通讯作者：
Katsura, Y
中科院分区：
文献类型：
--
作者：
Nishikawa, S;Nishikawa, S;Katsura, Y
我们研究了从胚胎中分离的内皮细胞（EC）和造血细胞（HPC）的淋巴造血潜能。用VE-钙粘蛋白、CD 45和Ter 119的表达来区分EC（VE-钙粘蛋白（+）CD 45（-）Ter 119（-））和HPC（VE-钙粘蛋白（-）CD 45（+））。因此，EC群体摄取乙酰化LDL并共表达CD 31、Flk 1和CD 34。在E9.5胚胎中，来自卵黄囊（YS）和胚胎本身的EC产生血细胞，包括淋巴细胞。因此，淋巴造血EC确实存在于胚胎中，并且它们在YS和胚胎本身中都产生。另一方面，具有淋巴细胞生成能力的HPC首先出现在胚胎中。这些研究结果暗示参与多种环境线索获得淋巴细胞生成能力在分化的HPC。
We have investigated the lymphohematopoietic potentials of endothelial cells (EC) and hematopoietic cells (HPC) sorted from embryos. Expression of VE-cadherin, CD45, and Ter119 was used to distinguish EC (VE-cadherin(+)CD45(-)Ter119(-)) from HPC (VE-cadherin(-)CD45(+)). Thus defined, EC population takes up acetylated LDL and coexpresses CD31, Flk1,and CD34. In E9.5 embryos, EC from yolk sac (YS) and the embryo proper generate blood cells, including lymphocytes. Thus, lymphohematopoietic EC do exist in the embryo, and they are generated both in YS and the embryo proper. On the other hand, HPC with lymphopoietic potency appear first in the embryo proper. These findings implicate involvement of multiple environmental cues for acquiring lymphopoietic competency during differentiation of HPC.