RELEASE OF NEURONOTROPHIC FACTOR FROM RABBIT CORNEAL EPITHELIUM DURING WOUND-HEALING AND NERVE REGENERATION
RELEASE OF NEURONOTROPHIC FACTOR FROM RABBIT CORNEAL EPITHELIUM DURING WOUND-HEALING AND NERVE REGENERATION
复制标题
DOI：
10.1016/s0014-4835(87)80112-4
复制
发表时间：
1987-11-01
影响因子：
3.4
通讯作者：
HASCHKE, RH
中科院分区：
文献类型：
--
作者：
CHAN, KY;JONES, RR;HASCHKE, RH
上皮神经营养因子(ENF)是由培养的兔角结膜上皮细胞分泌的一种神经营养因子，具有促进培养的三叉神经细胞存活和诱导突起生长的作用。本研究利用庚醇诱导的角膜上皮损伤模型，评价了ENF与角膜神经再生的关系。用器官培养技术从完整的角膜上皮细胞中收集ENF，并用神经元生物测定法对ENF进行定量。结果显示，无论是在最初的伤口闭合期间还是在1周后，当上皮再生时，ENF的分泌都没有变化。而伤后2周，ENF分泌量增加2.4倍。氯化金染色的角膜神经形态计量学分析显示，2周后可见上皮内神经首次再生，3周后上皮神经密度恢复正常。然而，伤后4周，损伤上皮的神经密度仍低于正常(比对照低35-47%)。因此，ENF分泌激增似乎发生在上皮再生之后，但在神经再生之前。结果提示，ENF可能参与了角膜神经的再生。
Epithelial neuronotropic factor (ENF) is secreted by cultured epithelial cells of rabbit cornea and conjunctiva, and is active in promoting survival and inducing neurite outgrowth of cultured trigeminal neurons. This study evaluated the relation of ENF to corneal nerve regeneration utilizing a model of heptanol-induced epithelial wounding. The organ culture technique was used to collect ENF from the intact corneal epithelium, and a neuronal bioassay was utilized to quantify ENF. The results revealed no change in ENF secretion either during initial wound closure or after 1 week, when the epithelium had regenerated. However, ENF secretion was elevated 2.4 times in 2 weeks after wounding. Morphometric analysis of corneal nerves stained by gold chloride impregnation showed that the first sign of regeneration of intraepithelial nerves was observed after 2 weeks, and the normal pattern of epithelial neural density was re-established after 3 weeks. However, the neural density was still subnormal (35-47% less than the control) in the wounded epithelium up to 4 weeks after wounding. Thus it appears that a surge in ENF secretion occurred after epithelial regeneration but before nerve regeneration. The results suggest that ENF may mediate corneal nerve regeneration.