Differential expression of aquaporin 5 and aquaporin 3 in squamous cell carcinoma and adenoid cystic carcinoma.
Differential expression of aquaporin 5 and aquaporin 3 in squamous cell carcinoma and adenoid cystic carcinoma.
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DOI：
10.3892/ijo.2012.1445
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发表时间：
2012-07
影响因子：
5.2
通讯作者：
S. Ishimoto;K. Wada;Y. Usami;N. Tanaka;T. Aikawa;M. Okura;A. Nakajima;M. Kogo;Y. Kamisaki
中科院分区：
文献类型：
--
作者：
S. Ishimoto;K. Wada;Y. Usami;N. Tanaka;T. Aikawa;M. Okura;A. Nakajima;M. Kogo;Y. Kamisaki
水通道蛋白 (AQP) 是一种膜蛋白家族，参与水跨细胞膜的选择性运输。最近的研究报道了 AQP5 在多种肿瘤类型中的表达，如胃癌、肺癌、卵巢癌、胰腺癌和结直肠癌。我们之前报道过AQP3在舌癌肿瘤细胞上的表达以及AQP3对细胞生长的重要作用。然而，人们对 AQP5 在舌鳞状细胞癌 (SCC) 中的表达和确切作用知之甚少。我们研究了 AQP5 和 AQP3 在人口腔 SCC 和腺样囊性癌 (ACC) 中的表达。通过免疫组织化学方法在 SCC 的肿瘤细胞上观察到 AQP5 和 AQP3 的过度表达，而 ACC 细胞则被这些针对 AQP 的抗体微弱染色。用泛 AQP 抑制剂或特异性 AQP5-siRNA 治疗显示，通过抑制整合素和丝裂原激活蛋白激酶途径，可抑制 SCC 细胞系的细胞生长。 AQP 在 SCC 而非 ACC 的细胞生长中发挥重要作用。
Aquaporins (AQPs) are a membrane protein family involved in the selective transport of water across cell membranes. Recent studies have reported the expression of AQP5 in several tumor types such as gastric, pulmonary, ovarian, pancreatic and colorectal cancer. We have previously reported the expression on tumor cells and the important role of AQP3 on cell growth in tongue cancer. However, little is known about the expression and precise role of AQP5 on squamous cell carcinoma (SCC) of the tongue. We investigated the expression of AQP5 and AQP3 in human oral SCC and adenoid cystic carcinoma (ACC). Overexpression of both AQP5 and AQP3 were immunohistochemically observed on tumor cells in SCC, whereas ACC cells were faintly stained with those antibodies against AQPs. Treatment with pan-AQP inhibitor or specific AQP5-siRNA showed inhibition of cell growth in SCC cell lines via the inhibition of integrins and the mitogen-activated protein kinase pathway. AQPs play important roles in cell growth in SCC rather than ACC.