Molecular characterization of a Babesia species identified in a North American raccoon
Molecular characterization of a Babesia species identified in a North American raccoon
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DOI：
10.7589/0090-3558-42.2.375
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发表时间：
2006-04-01
影响因子：
1.3
通讯作者：
Breitschwerdt, Edward B.
中科院分区：
文献类型：
--
作者：
Birkenheuer, Adam J.;Whittington, Julia;Breitschwerdt, Edward B.
梨形虫病于1926年首次在浣熊（Frocyon lotor）中被描述，1981年官方将一种小型梨形虫描述为巴氏梨形虫。在北美和日本的浣熊中已经发现了类似Bababelymicroti的基因序列。据文献记载，显微镜下的外观并不总是准确地预测基因型和系统发育分类。在评估小Piroplasty时，最常报告使用表型预测基因型的不确定性。我们从一只浣熊的小梨质体中扩增并测序了全长18S rRNA基因，并将此序列用于系统发育分析。基于这些分析，该生物体被置于严格意义上的巴贝虫分支中，确认它是一种真正的巴贝虫。可以感染浣熊本研究中产生的数据可用于设计用于检测这种Babysp.的分子诊断测试，这将有助于流行病学和比较系统发育研究。由于近年来梨形虫病在人类中的发生频率越来越高，本研究的结果将有助于识别人畜共患巴贝虫病。
Piroplasmosis was first described in raccoons (Frocyon lotor) in 1926, and the official description of a small piroplasm as Babesia lotori was done in 1981. Babesia microti-like gene sequences have been characterized in raccoons in both North American and Japan. It is well documented that the microscopic appearance of piroplasms does not always accurately predict the genotype and phylogenetic classification. Discrepancies using phenotype to predict genotype have been reported most frequently when evaluating small piroplasms. We amplified and sequenced the full-length 18S rRNA gene from a small piroplasm identified in a raccoon and used this sequence for phylogenetic analyses. Based on these analyses, the organism was placed in the Babesia sensu stricto clade, confirming that it is a true Babesia sp. This documents that at least two Babesia spp. can infect raccoons. The data generated in this study can be used to design molecular diagnostic tests for detection of this Babesia sp., which will be useful for epidemiologic and comparative phylogenetic studies. As piroplasmosis has been documented with increased frequency in humans in recent years, the results of this study will aid in the recognition of zoonotic babesiosis.