PLANT NATRIURETIC PEPTIDE A and Its Putative Receptor PNP-R2 Antagonize Salicylic Acid-Mediated Signaling and Cell Death
PLANT NATRIURETIC PEPTIDE A and Its Putative Receptor PNP-R2 Antagonize Salicylic Acid-Mediated Signaling and Cell Death
复制标题
植物利尿钠肽 A 及其推定受体 PNP-R2 拮抗水杨酸介导的信号传导和细胞死亡
DOI：
10.1105/tpc.20.00018
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发表时间：
2020-07-01
期刊：
影响因子：
11.6
通讯作者：
Kim, Chanhong
中科院分区：
文献类型：
--
作者：
Lee, Keun Pyo;Liu, Kaiwei;Kim, Chanhong
PNP-A及其受体蛋白PNP-R2介导的细胞间信号传导可抵消SA介导的植物胁迫反应。植物逆境激素水杨酸（SA）参与局部和系统获得性抗性，最终导致全株对细菌病原体的抗性。然而，如果SA介导的信号传导没有得到适当的控制，植物会产生与防御相关的适应性成本，如生长抑制和细胞死亡。尽管它的重要性，迄今为止，只有少数组件抵消SA引发的应激反应已被确定在拟南芥（拟南芥）。这些包括其他植物激素，如茉莉酸和脱落酸，以及蛋白质，如病变模拟疾病1，一种转录辅助调节因子。在这里，我们描述了植物促钠尿肽A（PNP-A），脊椎动物心房利钠肽的功能类似物，似乎拮抗SA介导的植物应激反应。虽然PNP-A的缺失增强了SA介导的信号传导，但外源性应用合成PNP-A或过量表达PNP-A显著损害了SA引发的免疫应答。此外，我们确定了质膜定位的受体样蛋白，PNP-R2，与PNP-A相互作用，并需要启动PNP-A介导的细胞内信号。总之，我们的工作确定了一个肽和它的推定同源受体作为抵消SA介导的信号和SA引发的细胞死亡在拟南芥。
Intercellular signaling mediated by PNP-A and its putative receptor protein PNP-R2 counteracts SA-mediated plant stress responses. The plant stress hormone salicylic acid (SA) participates in local and systemic acquired resistance, which eventually leads to whole-plant resistance to bacterial pathogens. However, if SA-mediated signaling is not appropriately controlled, plants incur defense-associated fitness costs such as growth inhibition and cell death. Despite its importance, to date only a few components counteracting the SA-primed stress responses have been identified in Arabidopsis (Arabidopsis thaliana). These include other plant hormones such as jasmonic acid and abscisic acid, and proteins such as LESION SIMULATING DISEASE1, a transcription coregulator. Here, we describe PLANT NATRIURETIC PEPTIDE A (PNP-A), a functional analog to vertebrate atrial natriuretic peptides, that appears to antagonize the SA-mediated plant stress responses. While loss of PNP-A potentiates SA-mediated signaling, exogenous application of synthetic PNP-A or overexpression of PNP-A significantly compromises the SA-primed immune responses. Moreover, we identify a plasma membrane-localized receptor-like protein, PNP-R2, that interacts with PNP-A and is required to initiate the PNP-A-mediated intracellular signaling. In summary, our work identifies a peptide and its putative cognate receptor as counteracting both SA-mediated signaling and SA-primed cell death in Arabidopsis.