Isolation and Potential for Transmission of Mycobacterium bovis at Human-livestock-wildlife Interface of the Serengeti Ecosystem, Northern Tanzania.
Isolation and Potential for Transmission of Mycobacterium bovis at Human-livestock-wildlife Interface of the Serengeti Ecosystem, Northern Tanzania.
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DOI：
10.1111/tbed.12445
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发表时间：
2017-06
影响因子：
4.3
通讯作者：
Matee MI
中科院分区：
文献类型：
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作者：
Katale BZ;Mbugi EV;Siame KK;Keyyu JD;Kendall S;Kazwala RR;Dockrell HM;Fyumagwa RD;Michel AL;Rweyemamu M;Streicher EM;Warren RM;van Helden P;Matee MI
牛分枝杆菌是牛结核（bTB）的病原体，是一种具有公共卫生和兽医重要性的多宿主病原体。我们对在塞伦盖蒂生态系统人类-牲畜-野生动物界面分离的牛分枝杆菌进行了特征鉴定，以确定相互作用宿主物种之间跨物种传播的流行病学和风险。从472例结核病疑似患者的痰样本和606种家畜和野生动物的组织样本中提取分枝杆菌培养物的DNA。利用spoligotyping和分枝杆菌穿插重复单元-可变串联重复序列（MIRU - VNTR）在24个位点上对牛分枝杆菌分离株进行了鉴定。从培养样品中仅分离到5株牛分枝杆菌。Spoligotyping结果显示，从2只水牛（Syncerus caffer）和1只非洲果子狸（Civettictis civetta）分离的3株牛分枝杆菌属SB0133 spoligotype。这两个新菌株（AR1和AR2）分别被鉴定为SB2290和SB2289 spoligotype。临床症状与结核病相符的患者未检出牛支原体。在472例疑似结核患者的606份动物组织标本和痰液中，分别有43份（7.09%）和12份（2.9%）检出非结核分枝杆菌（NTM），其中20株为胞内分枝杆菌。未发现鸟分枝杆菌。野生牛支原体分离株与AR1和AR2株的spoligotype分别为45.2%和96.8%。这一发现表明野生动物和牛的bTB感染具有流行病学相关性。在24个MIRU‐VNTR基因座中，QUB 11b在牛分枝杆菌株中表现出最高的特异性。本研究中获得的新菌株在该地区以前没有报道过，但没有发现牛支原体最近在人、牲畜和野生动物之间跨物种传播的明确证据。
Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB), is a multihost pathogen of public health and veterinary importance. We characterized the M. bovis isolated at the human–livestock–wildlife interface of the Serengeti ecosystem to determine the epidemiology and risk of cross‐species transmission between interacting hosts species. DNA was extracted from mycobacterial cultures obtained from sputum samples of 472 tuberculosis (TB) suspected patients and tissue samples from 606 livestock and wild animal species. M. bovis isolates were characterized using spoligotyping and Mycobacterial Interspersed Repetitive Units‐Variable Tandem Repeats (MIRU‐VNTR) on 24 loci. Only 5 M. bovis were isolated from the cultured samples. Spoligotyping results revealed that three M. bovis isolates from two buffaloes (Syncerus caffer) and 1 African civet (Civettictis civetta) belonged to SB0133 spoligotype. The two novel strains (AR1 and AR2) assigned as spoligotype SB2290 and SB2289, respectively, were identified from indigenous cattle (Bos indicus). No M. bovis was detected from patients with clinical signs consistent with TB. Of the 606 animal tissue specimens and sputa of 472 TB‐suspected patients 43 (7.09%) and 12 (2.9%), respectively, yielded non‐tuberculous mycobacteria (NTM), of which 20 isolates were M. intracellulare. No M. avium was identified. M. bovis isolates from wildlife had 45.2% and 96.8% spoligotype pattern agreement with AR1 and AR2 strains, respectively. This finding indicates that bTB infections in wild animals and cattle were epidemiologically related. Of the 24 MIRU‐VNTR loci, QUB 11b showed the highest discrimination among the M. bovis strains. The novel strains obtained in this study have not been previously reported in the area, but no clear evidence for recent cross‐species transmission of M. bovis was found between human, livestock and wild animals.